© - UVic Genome BC Proteomics Centre

Gradient|UVic PeptideTracker

Peptide ID Primary Protein Peptide Sequence Special Residues
PEP20130129010 Complement C3 TGLQEVEVK K+8
Retention Time
 Percent Organic Solution
 Analytical information
 Gradient
12.6(min) 11.81 Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um
Gradient 10
Time [min] B [%] Flow [ml/min]
0.00 1.5 0.4
1.50 6.3 0.4
16.00 13.5 0.4
18.00 13.77 0.4
33.00 22.5 0.4
38.00 40.5 0.4
39.00 81 0.4
42.90 81 0.4
43.00 1.5 0.4
11.92(min) 12.75 Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um
Gradient 11
Time [min] B [%] Flow [ml/min]
0.00 3.00 0.4
1.50 7.00 0.4
16.00 15.00 0.4
18.00 15.30 0.4
33.00 25.00 0.4
38.00 45.00 0.4
39.00 90.00 0.4
42.90 90.00 0.4
43.00 3.00 0.4
12.28(min) 11.65 Agilent AdvanceBio Peptide Map 2.1 x 150mm 2.7um
Gradient 13
Time [min] B [%] Flow [ml/min]
0.00 2.70 0.4
1.50 6.30 0.4
16.00 13.50 0.4
18.00 13.80 0.4
33.00 22.50 0.4
38.00 40.50 0.4
39.00 81.00 0.4
42.90 81.00 0.4
43.00 2.70 0.4
11.59(min) 11.31 Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um
Gradient 3
Time [min] B [%] Flow [ml/min]
0.00 1.5 0.4
1.50 6.3 0.4
16.00 13.5 0.4
18.00 13.77 0.4
33.00 22.5 0.4
38.00 40.5 0.4
39.00 81 0.4
42.90 81 0.4
43.00 1.5 0.4
11.590(min) 11.31 Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um
Gradient 2
Time [min] B [%] Flow [ml/min]
0 1.5 0.4
1.5 6.3 0.4
16 13.5 0.4
18 13.77 0.4
33 22.5 0.4
38 40.5 0.4
39 81 0.4
42.9 81 0.4
43 1.5 0.4
12.786(min) 11.9 Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um
Gradient 7
Time [min] B [%] Flow [ml/min]
0 1.5 0.4
1.5 6.3 0.4
16 13.5 0.4
18 13.77 0.4
33 22.5 0.4
38 40.5 0.4
39 81 0.4
42.9 81 0.4
43 2.7 0.4
11.55(min) 11.29 Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um
Gradient 4
Time [min] B [%] Flow [ml/min]
0.00 2.70 0.4
1.50 6.30 0.4
16.00 13.50 0.4
18.00 13.80 0.4
33.00 22.50 0.4
38.00 40.50 0.4
39.00 81.00 0.4
42.90 81.00 0.4
43.00 2.70 0.4
12.786(min) 11.9 Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um
Gradient 6
Time [min] B [%] Flow [ml/min]
0 1.5 0.4
1.5 6.3 0.4
16 13.5 0.4
18 13.77 0.4
33 22.5 0.4
38 40.5 0.4
39 81 0.4
42.9 81 0.4
43 2.7 0.4
6.83(min) 12.58 Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um
Gradient 5
Time [min] B [%] Flow [ml/min]
0 2.7 0.4
2 9.9 0.4
15 17.1 0.4
22 26.1 0.4
25 40.5 0.4
27 81 0.4
29 81 0.4
30 2.7 0.4
11.92(min) 12.75 Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um
Gradient 1
Time [min] B [%] Flow [ml/min]
0.00 3.00 0.4
1.50 7.00 0.4
16.00 15.00 0.4
18.00 15.30 0.4
33.00 25.00 0.4
38.00 45.00 0.4
39.00 90.00 0.4
42.90 90.00 0.4
43.00 3.00 0.4