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Results|UVic PeptideTracker

You searched for: Sus scrofa

UniProtKB Accession Protein Gene Organism Peptide Sequence Concentration Range Concentration Range Peptide ID Special Residues Special Residues Molecular Weight GRAVY Score Transitions Transitions Retention Time Retention Time Percent Organic Solution Percent Organic Solution Gradients Gradinets AAA CZE
UniProtKB Accession Protein Gene Organism Peptide Sequence Concentration Range Concentration Range Peptide ID Special Residues Special Residues Molecular Weight GRAVY Score Transitions Transitions Retention Time Retention Time Percent Organic Solution Percent Organic Solution AAA CZE
Q19AZ8 Prothrombin F2 Sus scrofa YGFYTHVFR NA NA PEP20130311018

R+10

R+10 1198.57 -0.18

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|400.5345|9|Positive|y8++|10|5|3|518.7656|380|171504.0,Agilent6490|2|True|400.5345|9|Positive|y7++|10|5|3|490.2548|380|42844.0,Agilent6490|3|True|400.5345|9|Positive|y6++|10|5|3|416.7206|380|36323.0,Agilent6490|4|True|400.5345|13|Positive|y4|10|5|3|568.323|380|31373.0,Agilent6490|5|True|400.5345|13|Positive|y5|10|5|3|669.3706|380|30910.0,Agilent6490|1|False|397.2|9|Positive|y8++|10|5|3|513.76|380|171504.0,Agilent6490|2|False|397.2|9|Positive|y7++|10|5|3|485.25|380|42844.0,Agilent6490|3|False|397.2|9|Positive|y6++|10|5|3|411.72|380|36323.0,Agilent6490|4|False|397.2|13|Positive|y4|10|5|3|558.31|380|31373.0,Agilent6490|5|False|397.2|13|Positive|y5|10|5|3|659.36|380|30910.0 21.467(min) 21.467(min);21.30(min);21.400(min) 15.81 15.81;15.71;15.75 View Gradient 9,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|2.7|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.8|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 9.000000 95.80
P67937 Tropomyosin alpha-4 chain TPM4 Sus scrofa IQALQQQADEAEDR NA NA PEP20121106027

R+10

R+10 1623.76 -1.34

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|812.8942|33|Positive|b3|10|646|2|313.187|380|7558.0,Agilent6490|2|True|812.8942|45|Positive|b2|10|647|2|242.1499|380|6219.0,Agilent6490|3|True|812.8942|37|Positive|y3++|10|648|2|215.102|380|2876.0,Agilent6490|4|True|812.8942|37|Positive|b4++|10|649|2|213.6392|380|2676.0,Agilent6490|5|True|812.8942|33|Positive|y10|10|650|2|1199.5163|380|1912.0,Agilent6490|1|False|807.8942|33|Positive|b3|10|651|2|313.187|380|7558.0,Agilent6490|2|False|807.8942|45|Positive|b2|10|652|2|242.1499|380|6219.0,Agilent6490|3|False|807.8942|37|Positive|y3++|10|653|2|210.102|380|2876.0,Agilent6490|4|False|807.8942|37|Positive|b4++|10|654|2|213.6392|380|2676.0,Agilent6490|5|False|807.8942|33|Positive|y10|10|655|2|1189.5163|380|1912.0,Agilent6490|1|True|812.8942|33|Positive|b3|10|5|2|313.187|380|7558.0,Agilent6490|2|True|812.8942|45|Positive|b2|10|5|2|242.1499|380|6219.0,Agilent6490|3|True|812.8942|37|Positive|y3++|10|5|2|215.102|380|2876.0,Agilent6490|4|True|812.8942|37|Positive|b4++|10|5|2|213.6392|380|2676.0,Agilent6490|5|True|812.8942|33|Positive|y10|10|5|2|1199.5163|380|1912.0,Agilent6490|1|False|807.8942|33|Positive|b3|10|5|2|313.187|380|7558.0,Agilent6490|2|False|807.8942|45|Positive|b2|10|5|2|242.1499|380|6219.0,Agilent6490|3|False|807.8942|37|Positive|y3++|10|5|2|210.102|380|2876.0,Agilent6490|4|False|807.8942|37|Positive|b4++|10|5|2|213.6392|380|2676.0,Agilent6490|5|False|807.8942|33|Positive|y10|10|5|2|1189.5163|380|1912.0 10.680(min) 10.680(min);10.59(min);11.07(min) 10.86 10.86;10.81;12.28 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4 9.688000 44.50
Q29371 Triosephosphate isomerase TPI1 Sus scrofa FFVGGNWK NA NA PEP20121119014

K+8

K+8 961.48 0.09

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|5|True|481.7529|13|Positive|y7|10|5|2|815.429|380|1545.0,Agilent6490|4|True|481.7529|21|Positive|y2|10|5|2|341.2063|380|1771.0,Agilent6490|3|True|481.7529|9|Positive|y5|10|5|2|569.2922|380|6014.0,Agilent6490|2|True|481.7529|9|Positive|b2|10|5|2|295.1441|380|7650.0,Agilent6490|1|True|481.7529|9|Positive|y6|10|5|2|668.3606|380|10895.0,Agilent6490|5|False|477.745|13|Positive|y7|10|5|2|807.41|380|1545.0,Agilent6490|4|False|477.745|21|Positive|y2|10|5|2|333.19|380|1771.0,Agilent6490|3|False|477.745|9|Positive|y5|10|5|2|561.28|380|6014.0,Agilent6490|2|False|477.745|9|Positive|b2|10|5|2|295.1441|380|7650.0,Agilent6490|1|False|477.745|9|Positive|y6|10|5|2|660.35|380|10895.0 24.110(min) 24.110(min);24.79(min);23.805(min);24.79(min);23.81(min) 17.33 17.33;19.69;17.15;19.69;17.15 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 8,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.77|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 11,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 12,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.77|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 2.185000 100.00
Q5XLD3 Creatine kinase M-type CKM Sus scrofa LGSSEVEQVQLVVDGVK NA NA PEP999903080006

K+8

K+8 1792.95 0.28

Qtrap4000

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|598.6632|17|Positive|y7|10|5|3|737.4647|380|7468.0,Agilent6490|2|True|598.6632|9|Positive|b11++|10|5|3|585.8037|380|7058.0,Agilent6490|3|True|598.6632|17|Positive|y5|10|5|3|525.3122|380|6682.0,Agilent6490|4|True|598.6632|9|Positive|b9++|10|5|3|465.2324|380|5446.0,Agilent6490|5|True|598.6632|13|Positive|y6|10|5|3|624.3807|380|5194.0,Agilent6490|1|False|595.99|17|Positive|y7|10|5|3|729.45|380|7468.0,Agilent6490|2|False|595.99|9|Positive|b11++|10|5|3|585.8037|380|7058.0,Agilent6490|3|False|595.99|17|Positive|y5|10|5|3|517.3|380|6682.0,Agilent6490|4|False|595.99|9|Positive|b9++|10|5|3|465.2324|380|5446.0,Agilent6490|5|False|595.99|13|Positive|y6|10|5|3|616.37|380|5194.0;Instrument|Rank|Heavy|Q1|Q3|CollisionEnergy|DeclusteringPotential|FragmentIon|ParentChargeState|Time|CellExitPotential|Abundance,Qtrap4000|1|True|598.7|624.4|25|85|y6|3|20|21|0.0,Qtrap4000|2|True|598.7|737.5|25|85|y7|3|20|21|0.0,Qtrap4000|3|True|598.7|525.3|25|85|y5|3|20|21|0.0,Qtrap4000|4|True|598.7|830.4|25|85|b8|3|20|21|0.0,Qtrap4000|5|True|598.7|865.5|25|85|y8|3|20|21|0.0,Qtrap4000|1|False|596.0333|616.4|25|85|y6|3|20|21|0.0,Qtrap4000|2|False|596.0333|729.5|25|85|y7|3|20|21|0.0,Qtrap4000|3|False|596.0333|517.3|25|85|y5|3|20|21|0.0,Qtrap4000|4|False|596.0333|830.4|25|85|b8|3|20|21|0.0,Qtrap4000|5|False|596.0333|857.5|25|85|y8|3|20|21|0.0 31.51(min) 31.51(min);30.90(min);30.930(min) 24.04 24.04;21.28;21.3 View Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 7.370000 98.70
P68137 Actin, alpha skeletal muscle ACTA1 Sus scrofa VAPEEHPTLLTEAPLNPK NA NA PEP20121001017

K+8

K+8 1963.04 -0.54

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|4|True|655.358|49|Positive|y2|10|5|3|252.1803|380|163.0,Agilent6490|3|True|655.358|9|Positive|y5|10|5|3|576.3601|380|163.0,Agilent6490|5|True|655.358|21|Positive|y6|10|5|3|647.3972|380|144.0,Agilent6490|2|True|655.358|17|Positive|y16++|10|5|3|897.4804|380|257.0,Agilent6490|1|True|655.358|13|Positive|y16+++|10|5|3|598.6562|380|659.0,Agilent6490|4|False|652.6913|49|Positive|y2|10|5|3|244.1803|380|163.0,Agilent6490|3|False|652.6913|9|Positive|y5|10|5|3|568.3601|380|163.0,Agilent6490|5|False|652.6913|21|Positive|y6|10|5|3|639.3972|380|144.0,Agilent6490|2|False|652.6913|17|Positive|y16++|10|5|3|893.4804|380|257.0,Agilent6490|1|False|652.6913|13|Positive|y16+++|10|5|3|595.9895|380|659.0 24.82(min) 24.82(min);24.31(min);24.82(min);24.320(min) 19.71 19.71;17.46;19.71;17.45 View Gradient 11,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 9.002000 98.47
Q71LE2 Histone H3.3 H3-3A Sus scrofa SAPSTGGVKKPHR NA NA PEP20121106031

R+10

R+10 1330.73 -1.22

NA

NA NA NA NA NA NA NA 9.523000 36.10
Q29221 F-actin-capping protein subunit alpha-2 CAPZA2 Sus scrofa LLLNNDNLLR NA NA PEP20120911023

R+10

R+10 1206.69 0.05

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|604.3558|5|Positive|b2|10|5|2|227.176|380|7959.0,Agilent6490|3|True|604.3558|37|Positive|y4|10|5|2|525.3388|380|951.0,Agilent6490|5|True|604.3558|21|Positive|y6|10|5|2|754.4087|380|679.0,Agilent6490|4|True|604.3558|25|Positive|y7|10|5|2|868.4516|380|717.0,Agilent6490|2|True|604.3558|17|Positive|y8++|10|5|2|491.2718|380|2340.0,Agilent6490|1|False|599.3558|5|Positive|b2|10|5|2|227.176|380|7959.0,Agilent6490|3|False|599.3558|37|Positive|y4|10|5|2|515.3388|380|951.0,Agilent6490|5|False|599.3558|21|Positive|y6|10|5|2|744.4087|380|679.0,Agilent6490|4|False|599.3558|25|Positive|y7|10|5|2|858.4516|380|717.0,Agilent6490|2|False|599.3558|17|Positive|y8++|10|5|2|486.2718|380|2340.0 27.390(min) 27.390(min);28.03(min);27.40(min);28.30(min) 19.23 19.23;21.79;19.25;19.77 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 13,column: Agilent AdvanceBio Peptide Map 2.1 x 150mm 2.7um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 7.785000 98.80
Q9GLP1 Coagulation factor V F5 Sus scrofa GEYEEHLGILGPIIR NA NA PEP20121015028

R+10

R+10 1704.9 -0.08

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|569.3103|13|Positive|y13++|10|5|3|760.429|380|29444.0,Agilent6490|2|True|569.3103|17|Positive|y5|10|5|3|565.3696|380|19726.0,Agilent6490|3|True|569.3103|9|Positive|y6++|10|5|3|339.7305|380|10209.0,Agilent6490|4|True|569.3103|9|Positive|b10++|10|5|3|571.2798|380|9921.0,Agilent6490|5|True|569.3103|9|Positive|y4++|10|5|3|254.6777|380|9531.0,Agilent6490|1|False|565.9737|13|Positive|y13++|10|5|3|755.425|380|29444.0,Agilent6490|2|False|565.9737|17|Positive|y5|10|5|3|555.361|380|19726.0,Agilent6490|3|False|565.9737|9|Positive|y6++|10|5|3|334.826|380|10209.0,Agilent6490|4|False|565.9737|9|Positive|b10++|10|5|3|571.28|380|9921.0,Agilent6490|5|False|565.9737|9|Positive|y4++|10|5|3|249.673|380|9531.0 31.58(min) 31.58(min);32.990(min);32.29(min);31.590(min);32.990(min);32.6(min);32.29(min) 21.68 21.68;22.49;24.54;21.68;22.49;22.27;24.54 View Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 7,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 6,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 10,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|1.5|0.4, 1.50|6.3|0.4, 16.00|13.5|0.4, 18.00|13.77|0.4, 33.00|22.5|0.4, 38.00|40.5|0.4, 39.00|81|0.4, 42.90|81|0.4, 43.00|1.5|0.4;Gradient 11,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4 8.641000 93.29
P24853 Insulin-like growth factor-binding protein 2 IGFBP2 Sus scrofa HHLGLEEPK NA NA PEP2013040219

K+8

K+8 1066.55 -1.30

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|356.5295|13|Positive|y2|10|5|3|252.1798|380|4105.0,Agilent6490|2|True|356.5295|9|Positive|b2|10|5|3|275.1251|380|3101.0,Agilent6490|3|True|356.5295|5|Positive|b7++|10|5|3|408.7036|380|1588.0,Agilent6490|4|True|356.5295|9|Positive|b4|10|5|3|445.2306|380|1549.0,Agilent6490|5|True|356.5295|9|Positive|y4|10|5|3|510.265|380|687.0,Agilent6490|1|False|353.86|13|Positive|y2|10|5|3|244.17|380|4105.0,Agilent6490|2|False|353.86|9|Positive|b2|10|5|3|275.1251|380|3101.0,Agilent6490|3|False|353.86|5|Positive|b7++|10|5|3|408.7036|380|1588.0,Agilent6490|4|False|353.86|9|Positive|b4|10|5|3|445.2306|380|1549.0,Agilent6490|5|False|353.86|9|Positive|y4|10|5|3|502.25|380|687.0 5.400(min) 5.400(min);6.302(min);5.35(min);6.302(min);6.20(min);6.1(min) 8.24 8.24;8.68;8.21;8.68;8.63;8.58 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 7,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 6,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 13,column: Agilent AdvanceBio Peptide Map 2.1 x 150mm 2.7um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 10,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|1.5|0.4, 1.50|6.3|0.4, 16.00|13.5|0.4, 18.00|13.77|0.4, 33.00|22.5|0.4, 38.00|40.5|0.4, 39.00|81|0.4, 42.90|81|0.4, 43.00|1.5|0.4 8.000000 100.00
Q08092 Calponin-1 CNN1 Sus scrofa GPAYGLSAEVK NA NA PEP2014031911

K+8

K+8 1098.57 -0.03

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|550.2979|17|Positive|y7|10|5|2|711.4127|380|16713.0,Agilent6490|2|True|550.2979|17|Positive|b3|10|5|2|226.1186|380|14539.0,Agilent6490|3|True|550.2979|17|Positive|y8|10|5|2|874.476|380|12256.0,Agilent6490|4|True|550.2979|17|Positive|y5|10|5|2|541.3072|380|7696.0,Agilent6490|5|True|550.2979|17|Positive|y9|10|5|2|945.5131|380|7644.0,Agilent6490|1|False|546.29|17|Positive|y7|10|5|2|703.4|380|16713.0,Agilent6490|2|False|546.29|17|Positive|b3|10|5|2|226.1186|380|14539.0,Agilent6490|3|False|546.29|17|Positive|y8|10|5|2|866.46|380|12256.0,Agilent6490|4|False|546.29|17|Positive|y5|10|5|2|533.29|380|7696.0,Agilent6490|5|False|546.29|17|Positive|y9|10|5|2|937.5|380|7644.0 14.220(min) 14.220(min) 12.62 12.62 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 10.000000 94.68
P32752 Cholinesterase BCHE Sus scrofa NIAAFGGNPK NA NA PEP20121001014

K+8

K+8 995.51 -0.24

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|498.7718|9|Positive|b2|10|5|2|228.1343|380|41795.0,Agilent6490|3|True|498.7718|25|Positive|y2|10|5|2|252.1798|380|12418.0,Agilent6490|5|True|498.7718|13|Positive|b3|10|5|2|299.1714|380|8784.0,Agilent6490|4|True|498.7718|13|Positive|y7|10|5|2|698.3712|380|9482.0,Agilent6490|2|True|498.7718|9|Positive|y8|10|5|2|769.4083|380|40046.0,Agilent6490|1|False|494.7718|9|Positive|b2|10|5|2|228.1343|380|41795.0,Agilent6490|3|False|494.7718|25|Positive|y2|10|5|2|244.1798|380|12418.0,Agilent6490|5|False|494.7718|13|Positive|b3|10|5|2|299.1714|380|8784.0,Agilent6490|4|False|494.7718|13|Positive|y7|10|5|2|690.3712|380|9482.0,Agilent6490|2|False|494.7718|9|Positive|y8|10|5|2|761.4083|380|40046.0 11.60(min) 11.60(min);11.230(min);11.19(min) 12.57 12.57;11.13;11.11 View Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 12.842000 99.30
P06867 Plasminogen PLG Sus scrofa HSIFTPETNPR NA NA PEP2013090637

R+10

R+10 1307.64 -1.16

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|436.8911|21|Positive|b2|10|5|3|225.0988|380|1683.0,Agilent6490|2|True|436.8911|9|Positive|b4|10|5|3|485.2512|380|787.0,Agilent6490|3|True|436.8911|9|Positive|y4|10|5|3|497.2711|380|700.0,Agilent6490|4|True|436.8911|21|Positive|b3|10|5|3|338.1828|380|659.0,Agilent6490|5|True|436.8911|5|Positive|y10++|10|5|3|586.3033|380|619.0,Agilent6490|1|False|433.5578|21|Positive|b2|10|5|3|225.0988|380|1683.0,Agilent6490|2|False|433.5578|9|Positive|b4|10|5|3|485.2512|380|787.0,Agilent6490|3|False|433.5578|9|Positive|y4|10|5|3|487.2711|380|700.0,Agilent6490|4|False|433.5578|21|Positive|b3|10|5|3|338.1828|380|659.0,Agilent6490|5|False|433.5578|5|Positive|y10++|10|5|3|581.3033|380|619.0 NA NA NA NA NA NA NA NA
P28491 Calreticulin CALR Sus scrofa VHVIFNYK NA NA PEP20130306026

K+8

K+8 1026.56 0.48

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|514.2949|17|Positive|b2|10|5|2|237.1346|380|10734.0,Agilent6490|2|True|514.2949|13|Positive|y7++|10|5|2|464.7602|380|8512.0,Agilent6490|3|True|514.2949|17|Positive|y6|10|5|2|791.4542|380|8105.0,Agilent6490|4|True|514.2949|13|Positive|b3|10|5|2|336.203|380|3636.0,Agilent6490|5|True|514.2949|25|Positive|y2|10|5|2|318.1903|380|2879.0,Agilent6490|1|False|510.29|17|Positive|b2|10|5|2|237.1346|380|10734.0,Agilent6490|2|False|510.29|13|Positive|y7++|10|5|2|460.75|380|8512.0,Agilent6490|3|False|510.29|17|Positive|y6|10|5|2|783.44|380|8105.0,Agilent6490|4|False|510.29|13|Positive|b3|10|5|2|336.203|380|3636.0,Agilent6490|5|False|510.29|25|Positive|y2|10|5|2|310.18|380|2879.0 15.59(min) 15.59(min);15.660(min) 13.3 13.3;13.33 View Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 8.000000 100.00
P67937 Tropomyosin alpha-4 chain TPM4 Sus scrofa SLEAASEK NA NA PEP20121106041

K+8

K+8 841.41 -0.64

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|421.7215|9|Positive|b4++|10|5|2|201.1052|380|31571.0,Agilent6490|2|True|421.7215|9|Positive|y6|10|5|2|642.3184|380|17133.0,Agilent6490|3|True|421.7215|13|Positive|y5|10|5|2|513.2759|380|5117.0,Agilent6490|4|True|421.7215|17|Positive|y3|10|5|2|371.2016|380|3656.0,Agilent6490|5|True|421.7215|9|Positive|b3|10|5|2|330.166|380|2378.0,Agilent6490|1|False|417.7138|9|Positive|b4++|10|5|2|201.105|380|31571.0,Agilent6490|2|False|417.7138|9|Positive|y6|10|5|2|634.304|380|17133.0,Agilent6490|3|False|417.7138|13|Positive|y5|10|5|2|505.261|380|5117.0,Agilent6490|4|False|417.7138|17|Positive|y3|10|5|2|363.187|380|3656.0,Agilent6490|5|False|417.7138|9|Positive|b3|10|5|2|330.167|380|2378.0 2.93(min) 2.93(min);3.21(min);3.220(min) 7.79 7.79;7.15;7.15 View Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 11.424000 52.20
Q29594 Creatine kinase B-type CKB Sus scrofa LAVEALSSLDGDLAGR NA NA PEP2013020506

R+10

R+10 1595.83 0.46

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|798.9275|25|Positive|y10|10|5|2|1000.4933|380|247229.0,Agilent6490|2|True|798.9275|33|Positive|y9|10|5|2|913.4613|380|142720.0,Agilent6490|3|True|798.9275|41|Positive|y4|10|5|2|426.2699|380|120288.0,Agilent6490|4|True|798.9275|41|Positive|y6|10|5|2|598.3183|380|119022.0,Agilent6490|5|True|798.9275|21|Positive|b5|10|5|2|484.2766|380|106762.0,Agilent6490|1|False|793.92|25|Positive|y10|10|5|2|990.48|380|247229.0,Agilent6490|2|False|793.92|33|Positive|y9|10|5|2|903.45|380|142720.0,Agilent6490|3|False|793.92|41|Positive|y4|10|5|2|416.26|380|120288.0,Agilent6490|4|False|793.92|41|Positive|y6|10|5|2|588.31|380|119022.0,Agilent6490|5|False|793.92|21|Positive|b5|10|5|2|484.2766|380|106762.0 32.550(min) 32.550(min);33.22(min);32.55(min) 22.24 22.24;25.88;22.24 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 7.940000 98.70
O19004 Serine/threonine-protein kinase A-Raf ARAF Sus scrofa IGDFGLATVK NA NA PEP2014012807

K+8

K+8 1027.57 0.82

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|514.7975|9|Positive|y9|10|5|2|915.5026|380|80645.0,Agilent6490|2|True|514.7975|13|Positive|y8|10|5|2|858.4811|380|46711.0,Agilent6490|3|True|514.7975|13|Positive|y6|10|5|2|596.3857|380|37517.0,Agilent6490|4|True|514.7975|13|Positive|b3|10|5|2|286.1397|380|32457.0,Agilent6490|5|True|514.7975|13|Positive|y7|10|5|2|743.4542|380|26188.0,Agilent6490|1|False|510.79|9|Positive|y9|10|5|2|907.49|380|80645.0,Agilent6490|2|False|510.79|13|Positive|y8|10|5|2|850.47|380|46711.0,Agilent6490|3|False|510.79|13|Positive|y6|10|5|2|588.37|380|37517.0,Agilent6490|4|False|510.79|13|Positive|b3|10|5|2|286.1397|380|32457.0,Agilent6490|5|False|510.79|13|Positive|y7|10|5|2|735.44|380|26188.0 NA NA NA NA NA NA 10.000000 93.10
P53027 60S ribosomal protein L10a RPL10A Sus scrofa EVLHGNQR NA NA PEP2013062014

R+10

R+10 961.49 -1.32

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|321.5068|9|Positive|y5++|10|5|3|311.1582|380|21240.0,Agilent6490|2|True|321.5068|9|Positive|y4|10|5|3|484.2502|380|16500.0,Agilent6490|3|True|321.5068|5|Positive|y6++|10|5|3|367.7002|380|11805.0,Agilent6490|4|True|321.5068|9|Positive|y2|10|5|3|313.1858|380|2198.0,Agilent6490|5|True|321.5068|9|Positive|y5|10|5|3|621.3091|380|2151.0,Agilent6490|1|False|318.17|9|Positive|y5++|10|5|3|306.15|380|21240.0,Agilent6490|2|False|318.17|9|Positive|y4|10|5|3|474.24|380|16500.0,Agilent6490|3|False|318.17|5|Positive|y6++|10|5|3|362.7|380|11805.0,Agilent6490|4|False|318.17|9|Positive|y2|10|5|3|303.18|380|2198.0,Agilent6490|5|False|318.17|9|Positive|y5|10|5|3|611.3|380|2151.0 2.55(min) 2.55(min);2.550(min) 6.82 6.82;6.82 View Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 10.000000 100.00
Q29411 Chitinase-3-like protein 1 CHI3L1 Sus scrofa TLLSVGGWNFGSQR NA NA PEP20121119021

R+10

R+10 1530.77 -0.09

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|766.3988|29|Positive|b2|10|5|2|215.139|380|248324.0,Agilent6490|2|True|766.3988|29|Positive|y9|10|5|2|1018.4729|380|183715.0,Agilent6490|3|True|766.3988|25|Positive|y8|10|5|2|961.4514|380|60802.0,Agilent6490|4|True|766.3988|21|Positive|y12++|10|5|2|659.3323|380|58242.0,Agilent6490|5|True|766.3988|25|Positive|b3|10|5|2|328.2231|380|51127.0,Agilent6490|1|False|761.3988|29|Positive|b2|10|5|2|215.139|380|248324.0,Agilent6490|2|False|761.3988|29|Positive|y9|10|5|2|1008.4729|380|183715.0,Agilent6490|3|False|761.3988|25|Positive|y8|10|5|2|951.4514|380|60802.0,Agilent6490|4|False|761.3988|21|Positive|y12++|10|5|2|654.3323|380|58242.0,Agilent6490|5|False|761.3988|25|Positive|b3|10|5|2|328.2231|380|51127.0 36.160(min) 36.160(min) 33.88 33.88 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 5.000000 88.73
O02705 Heat shock protein HSP 90-alpha HSP90AA1 Sus scrofa HSQFIGYPITLFVEK NA NA PEP2013030602

K+8

K+8 1785.94 0.25

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|596.326|9|Positive|y8++|10|5|3|477.7911|380|9032.0,Agilent6490|2|True|596.326|9|Positive|y8|10|5|3|954.575|380|3491.0,Agilent6490|3|True|596.326|13|Positive|b6|10|5|3|670.3307|380|3481.0,Agilent6490|4|True|596.326|9|Positive|b7|10|5|3|833.3941|380|2291.0,Agilent6490|5|True|596.326|13|Positive|y6|10|5|3|744.4382|380|2207.0,Agilent6490|1|False|593.654|9|Positive|y8++|10|5|3|473.78|380|9032.0,Agilent6490|2|False|593.654|9|Positive|y8|10|5|3|946.56|380|3491.0,Agilent6490|3|False|593.654|13|Positive|b6|10|5|3|670.3307|380|3481.0,Agilent6490|4|False|593.654|9|Positive|b7|10|5|3|833.3941|380|2291.0,Agilent6490|5|False|593.654|13|Positive|y6|10|5|3|736.42|380|2207.0 35.660(min) 35.660(min);35.68(min) 32.08 32.08;32.15 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 9.000000 75.00
P20305 Gelsolin GSN Sus scrofa AGALNSNDAFVLK NA NA PEP2016112411

K+8

K+8 1326.69 0.34

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|664.359|21|Positive|b3|10|5|2|200.1035|380|1467.0,Agilent6490|3|True|664.359|13|Positive|b4|10|5|2|313.1876|380|352.0,Agilent6490|5|True|664.359|17|Positive|y4|10|5|2|514.3484|380|250.0,Agilent6490|2|True|664.359|29|Positive|y2|10|5|2|268.2116|380|649.0,Agilent6490|4|True|664.359|25|Positive|y3|10|5|2|367.28|380|325.0,Agilent6490|1|False|660.359|21|Positive|b3|10|5|2|200.1035|380|1467.0,Agilent6490|3|False|660.359|13|Positive|b4|10|5|2|313.1876|380|352.0,Agilent6490|5|False|660.359|17|Positive|y4|10|5|2|506.3484|380|250.0,Agilent6490|2|False|660.359|29|Positive|y2|10|5|2|260.2116|380|649.0,Agilent6490|4|False|660.359|25|Positive|y3|10|5|2|359.28|380|325.0 NA NA NA NA NA NA 3.700000 93.68
Q29221 F-actin-capping protein subunit alpha-2 CAPZA2 Sus scrofa EGAAHAFAQYNLDQFTPVK NA NA PEP20120911096

K+8

K+8 2114.02 -0.41

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|2|True|705.6849|17|Positive|b9++|10|5|3|442.207|380|168.0,Agilent6490|3|True|705.6849|9|Positive|b10++|10|5|3|523.7387|380|147.0,Agilent6490|1|True|705.6849|33|Positive|y3|10|5|3|351.2487|380|226.0,Agilent6490|5|True|705.6849|17|Positive|y7|10|5|3|842.4503|380|134.0,Agilent6490|4|True|705.6849|13|Positive|y9++|10|5|3|535.2926|380|138.0,Agilent6490|2|False|703.0182|17|Positive|b9++|10|5|3|442.207|380|168.0,Agilent6490|3|False|703.0182|9|Positive|b10++|10|5|3|523.7387|380|147.0,Agilent6490|1|False|703.0182|33|Positive|y3|10|5|3|343.2487|380|226.0,Agilent6490|5|False|703.0182|17|Positive|y7|10|5|3|834.4503|380|134.0,Agilent6490|4|False|703.0182|13|Positive|y9++|10|5|3|531.2926|380|138.0 29.290(min) 29.290(min);29.94(min);29.25(min) 20.34 20.34;23.02;20.32 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 5.873000 95.10
Q08092 Calponin-1 CNN1 Sus scrofa VNVGVK NA NA PEP2013072414

K+8

K+8 622.38 0.80

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|312.2025|9|Positive|y5|10|5|2|524.3282|380|37928.0,Agilent6490|2|True|312.2025|13|Positive|b2|10|5|2|214.1186|380|20177.0,Agilent6490|3|True|312.2025|9|Positive|y4|10|5|2|410.2853|380|15415.0,Agilent6490|4|True|312.2025|13|Positive|y2|10|5|2|254.1954|380|8285.0,Agilent6490|5|True|312.2025|5|Positive|y4++|10|5|2|205.6463|380|8078.0,Agilent6490|1|False|308.2|9|Positive|y5|10|5|2|516.31|380|37928.0,Agilent6490|2|False|308.2|13|Positive|b2|10|5|2|214.1186|380|20177.0,Agilent6490|3|False|308.2|9|Positive|y4|10|5|2|402.27|380|15415.0,Agilent6490|4|False|308.2|13|Positive|y2|10|5|2|246.18|380|8285.0,Agilent6490|5|False|308.2|5|Positive|y4++|10|5|2|201.64|380|8078.0 4.40(min) 4.40(min);4.410(min) 7.74 7.74;7.74 View Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 5.000000 86.90
P02189 Myoglobin MB Sus scrofa GLSDGEWQLVLNVWGK NA NA PEP9999041737

K+8

K+8 1807.92 -0.12

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|904.9752|21|Positive|y3|10|5|2|398.2278|380|2520.0,Agilent6490|2|True|904.9752|33|Positive|y2|10|5|2|212.1485|380|1979.0,Agilent6490|3|True|904.9752|25|Positive|y6|10|5|2|724.4232|380|1726.0,Agilent6490|4|True|904.9752|29|Positive|y5|10|5|2|611.3391|380|1253.0,Agilent6490|5|True|904.9752|33|Positive|y8|10|5|2|936.5757|380|1155.0,Agilent6490|1|False|900.9676|21|Positive|y3|10|5|2|390.213|380|2520.0,Agilent6490|2|False|900.9676|33|Positive|y2|10|5|2|204.134|380|1979.0,Agilent6490|3|False|900.9676|25|Positive|y6|10|5|2|716.408|380|1726.0,Agilent6490|4|False|900.9676|29|Positive|y5|10|5|2|603.324|380|1253.0,Agilent6490|5|False|900.9676|33|Positive|y8|10|5|2|928.561|380|1155.0 37.070(min) 37.070(min) 37.15 37.15 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 11.330000 86.40
Q95332 Betaine--homocysteine S-methyltransferase 1 BHMT Sus scrofa AGPWTPEAAVEHPEAVR NA NA PEP2013040941

R+10

R+10 1825.89 -0.55

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|609.6407|13|Positive|y15+++|10|5|3|566.954|380|69174.0,Agilent6490|2|True|609.6407|13|Positive|y12++|10|5|3|657.8374|380|53984.0,Agilent6490|3|True|609.6407|17|Positive|y13++|10|5|3|708.3613|380|20157.0,Agilent6490|4|True|609.6407|21|Positive|y5|10|5|3|581.3281|380|17302.0,Agilent6490|5|True|609.6407|25|Positive|y9+++|10|5|3|339.8499|380|14666.0,Agilent6490|1|False|606.304|13|Positive|y15+++|10|5|3|563.62|380|69174.0,Agilent6490|2|False|606.304|13|Positive|y12++|10|5|3|652.83|380|53984.0,Agilent6490|3|False|606.304|17|Positive|y13++|10|5|3|703.36|380|20157.0,Agilent6490|4|False|606.304|21|Positive|y5|10|5|3|571.32|380|17302.0,Agilent6490|5|False|606.304|25|Positive|y9+++|10|5|3|336.51|380|14666.0 21.630(min) 21.630(min) 15.88 15.88 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 NA 75.25
P20112 SPARC SPARC Sus scrofa LEAGDHPVELLAR NA NA PEP9999032657

R+10

R+10 1428.75 -0.08

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|5|True|477.2612|17|Positive|y8++|10|5|3|472.7818|380|157.0,Agilent6490|4|True|477.2612|13|Positive|y10++|10|5|3|558.806|380|193.0,Agilent6490|1|True|477.2612|9|Positive|y11++|10|5|3|594.3245|380|1595.0,Agilent6490|2|True|477.2612|13|Positive|y12++|10|5|3|658.8458|380|487.0,Agilent6490|3|True|477.2612|17|Positive|y7|10|5|3|807.4968|380|208.0,Agilent6490|5|False|473.9279|17|Positive|y8++|10|5|3|467.7818|380|157.0,Agilent6490|4|False|473.9279|13|Positive|y10++|10|5|3|553.806|380|193.0,Agilent6490|1|False|473.9279|9|Positive|y11++|10|5|3|589.3245|380|1595.0,Agilent6490|2|False|473.9279|13|Positive|y12++|10|5|3|653.8458|380|487.0,Agilent6490|3|False|473.9279|17|Positive|y7|10|5|3|797.4968|380|208.0 19.050(min) 19.050(min);19.04(min);20.683(min);19.82(min);19.82(min);20.3(min);20.683(min) 14.38 14.38;14.4;15.33;16.48;16.48;15.11;15.33 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 6,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 11,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 10,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|1.5|0.4, 1.50|6.3|0.4, 16.00|13.5|0.4, 18.00|13.77|0.4, 33.00|22.5|0.4, 38.00|40.5|0.4, 39.00|81|0.4, 42.90|81|0.4, 43.00|1.5|0.4;Gradient 7,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4 7.590000 98.10
Q28833 von Willebrand factor VWF Sus scrofa EGGPSQIGDALGFAVR NA NA PEP20121022034

R+10

R+10 1582.79 -0.01

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|792.4068|29|Positive|y9|10|5|2|915.4922|380|76392.0,Agilent6490|2|True|792.4068|29|Positive|y10|10|5|2|1028.5763|380|41447.0,Agilent6490|3|True|792.4068|25|Positive|y5|10|5|2|559.3226|380|24377.0,Agilent6490|4|True|792.4068|45|Positive|y7|10|5|2|743.4438|380|20430.0,Agilent6490|5|True|792.4068|29|Positive|y13++|10|5|2|670.8635|380|15229.0,Agilent6490|1|False|787.4027|29|Positive|y9|10|5|2|905.4922|380|76392.0,Agilent6490|2|False|787.4027|29|Positive|y10|10|5|2|1018.5763|380|41447.0,Agilent6490|3|False|787.4027|25|Positive|y5|10|5|2|549.3226|380|24377.0,Agilent6490|4|False|787.4027|45|Positive|y7|10|5|2|733.4438|380|20430.0,Agilent6490|5|False|787.4027|29|Positive|y13++|10|5|2|665.8635|380|15229.0 31.04(min) 31.04(min);30.990(min) 21.36 21.36;21.33 View Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 5.236000 100.00
P86412 Myelin P2 protein PMP2 Sus scrofa NTEISFK NA NA PEP2013080719

K+8

K+8 845.42 -0.73

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|423.7265|17|Positive|y3|10|5|2|389.2274|380|7673.0,Agilent6490|2|True|423.7265|5|Positive|b2|10|5|2|216.0979|380|6106.0,Agilent6490|3|True|423.7265|9|Positive|y5|10|5|2|631.3541|380|5534.0,Agilent6490|4|True|423.7265|13|Positive|y4|10|5|2|502.3115|380|4662.0,Agilent6490|5|True|423.7265|17|Positive|y2|10|5|2|302.1954|380|3369.0,Agilent6490|1|False|419.72|17|Positive|y3|10|5|2|381.21|380|7673.0,Agilent6490|2|False|419.72|5|Positive|b2|10|5|2|216.0979|380|6106.0,Agilent6490|3|False|419.72|9|Positive|y5|10|5|2|623.34|380|5534.0,Agilent6490|4|False|419.72|13|Positive|y4|10|5|2|494.3|380|4662.0,Agilent6490|5|False|419.72|17|Positive|y2|10|5|2|294.18|380|3369.0 10.410(min) 10.410(min);10.38(min);11.20(min) 10.72 10.72;10.71;11.12 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 13,column: Agilent AdvanceBio Peptide Map 2.1 x 150mm 2.7um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 9.000000 73.80
O18994 Epididymal secretory glutathione peroxidase GPX5 Sus scrofa GDVNGEK NA NA PEP20130209017

K+6

K+6 723.33 -1.57

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|4|True|362.6826|5|Positive|b3|10|5|2|272.1246|380|108.0,Agilent6490|3|True|362.6826|9|Positive|y3|10|5|2|339.1975|380|195.0,Agilent6490|5|True|362.6826|5|Positive|b4|10|5|2|386.1676|380|67.0,Agilent6490|1|True|362.6826|9|Positive|y4|10|5|2|453.2405|380|337.0,Agilent6490|2|True|362.6826|9|Positive|y5|10|5|2|552.3089|380|289.0,Agilent6490|4|False|359.6826|5|Positive|b3|10|5|2|272.1246|380|108.0,Agilent6490|3|False|359.6826|9|Positive|y3|10|5|2|333.1975|380|195.0,Agilent6490|5|False|359.6826|5|Positive|b4|10|5|2|386.1676|380|67.0,Agilent6490|1|False|359.6826|9|Positive|y4|10|5|2|447.2405|380|337.0,Agilent6490|2|False|359.6826|9|Positive|y5|10|5|2|546.3089|380|289.0 1.470(min) 1.470(min);1.23(min) 6.2 6.2;5.65 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 6.420000 88.00
Q8SPS7 Haptoglobin HP Sus scrofa VGYVSGWGR NA NA PEP9999040974

R+10

R+10 989.49 -0.03

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|495.7557|13|Positive|y5|10|5|2|572.282|380|778.0,Agilent6490|2|True|495.7557|13|Positive|b3|10|5|2|320.161|380|511.0,Agilent6490|3|True|495.7557|17|Positive|y6|10|5|2|671.3505|380|286.0,Agilent6490|4|True|495.7557|9|Positive|y4|10|5|2|485.25|380|274.0,Agilent6490|5|True|495.7557|9|Positive|y7++|10|5|2|417.7108|380|256.0,Agilent6490|1|False|490.7557|13|Positive|y5|10|5|2|562.282|380|778.0,Agilent6490|2|False|490.7557|13|Positive|b3|10|5|2|320.161|380|511.0,Agilent6490|3|False|490.7557|17|Positive|y6|10|5|2|661.3505|380|286.0,Agilent6490|4|False|490.7557|9|Positive|y4|10|5|2|475.25|380|274.0,Agilent6490|5|False|490.7557|9|Positive|y7++|10|5|2|412.7108|380|256.0 NA NA NA NA NA NA 6.190000 54.00
Q52N47 Integral membrane protein 2B ITM2B Sus scrofa LTAYLDLNLDK NA NA PEP20130206012

K+8

K+8 1285.69 0.05

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|643.8583|17|Positive|y9|10|5|2|1072.5765|380|12356.0,Agilent6490|2|True|643.8583|17|Positive|b2|10|5|2|215.139|380|11042.0,Agilent6490|3|True|643.8583|17|Positive|y8|10|5|2|1001.5393|380|6362.0,Agilent6490|4|True|643.8583|21|Positive|y7|10|5|2|838.476|380|6216.0,Agilent6490|5|True|643.8583|21|Positive|y6|10|5|2|725.3919|380|5528.0,Agilent6490|1|False|639.85|17|Positive|y9|10|5|2|1064.56|380|12356.0,Agilent6490|2|False|639.85|17|Positive|b2|10|5|2|215.139|380|11042.0,Agilent6490|3|False|639.85|17|Positive|y8|10|5|2|993.52|380|6362.0,Agilent6490|4|False|639.85|21|Positive|y7|10|5|2|830.46|380|6216.0,Agilent6490|5|False|639.85|21|Positive|y6|10|5|2|717.38|380|5528.0 29.760(min) 29.760(min);29.74(min) 20.61 20.61;20.61 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 10.000000 90.80
P16545 Insulin-like growth factor I IGF1 Sus scrofa GFYFNKPTGYGSSSR NA NA PEP2013021502

R+10

R+10 1676.77 -0.99

Qtrap4000

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|3|True|559.9352|9|Positive|y13+++|10|5|3|491.9053|380|1097.0,Agilent6490|5|True|559.9352|17|Positive|y5|10|5|3|503.2453|380|444.0,Agilent6490|4|True|559.9352|13|Positive|y11++|10|5|3|582.2881|380|556.0,Agilent6490|2|True|559.9352|13|Positive|y12++|10|5|3|655.8223|380|1322.0,Agilent6490|1|True|559.9352|13|Positive|y13++|10|5|3|737.354|380|1547.0,Agilent6490|3|False|556.6019|9|Positive|y13+++|10|5|3|488.572|380|1097.0,Agilent6490|5|False|556.6019|17|Positive|y5|10|5|3|493.2453|380|444.0,Agilent6490|4|False|556.6019|13|Positive|y11++|10|5|3|577.2881|380|556.0,Agilent6490|2|False|556.6019|13|Positive|y12++|10|5|3|650.8223|380|1322.0,Agilent6490|1|False|556.6019|13|Positive|y13++|10|5|3|732.354|380|1547.0;Instrument|Rank|Heavy|Q1|Q3|CollisionEnergy|DeclusteringPotential|FragmentIon|ParentChargeState|Time|CellExitPotential|Abundance,Qtrap4000|1|True|559.9|737.35|23|62|y++13|3|20|21|0.0,Qtrap4000|2|True|559.9|655.8|23|62|y++12|3|20|21|0.0,Qtrap4000|3|True|559.9|205.1|25|62|b2|3|20|21|0.0,Qtrap4000|4|True|559.9|921.4|33|62|y9|3|20|21|0.0,Qtrap4000|5|True|559.9|503.2|26|62|y5|3|20|21|0.0,Qtrap4000|1|False|556.5667|732.35|23|62|y++13|3|20|21|0.0,Qtrap4000|2|False|556.5667|650.8|23|62|y++12|3|20|21|0.0,Qtrap4000|3|False|556.5667|205.1|25|62|b2|3|20|21|0.0,Qtrap4000|4|False|556.5667|911.4|33|62|y9|3|20|21|0.0,Qtrap4000|5|False|556.5667|493.2|26|62|y5|3|20|21|0.0 17.653(min) 17.653(min);16.644(min);17.15(min);17.400(min);17.653(min);17.4(min) 13.72 13.72;13.6;15.17;13.69;13.72;13.69 View Gradient 6,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 9,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|2.7|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.8|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 7,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 10,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|1.5|0.4, 1.50|6.3|0.4, 16.00|13.5|0.4, 18.00|13.77|0.4, 33.00|22.5|0.4, 38.00|40.5|0.4, 39.00|81|0.4, 42.90|81|0.4, 43.00|1.5|0.4 5.700000 97.00
P24853 Insulin-like growth factor-binding protein 2 IGFBP2 Sus scrofa LRPPPAR NA NA PEP2012110602

R+10

R+10 815.49 -1.17

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|408.7581|17|Positive|y5|10|246|2|547.3226|380|121071.0,Agilent6490|2|True|408.7581|17|Positive|b2|10|247|2|270.1925|380|50991.0,Agilent6490|3|True|408.7581|17|Positive|y4++|10|248|2|225.6386|380|35244.0,Agilent6490|4|True|408.7581|21|Positive|y4|10|249|2|450.2699|380|32887.0,Agilent6490|5|True|408.7581|17|Positive|y5++|10|250|2|274.165|380|26956.0,Agilent6490|1|False|403.7581|17|Positive|y5|10|251|2|537.3226|380|121071.0,Agilent6490|2|False|403.7581|17|Positive|b2|10|252|2|270.1925|380|50991.0,Agilent6490|3|False|403.7581|17|Positive|y4++|10|253|2|220.6386|380|35244.0,Agilent6490|4|False|403.7581|21|Positive|y4|10|254|2|440.2699|380|32887.0,Agilent6490|5|False|403.7581|17|Positive|y5++|10|255|2|269.165|380|26956.0,Agilent6490|1|True|408.7581|17|Positive|y5|10|5|2|547.3226|380|121071.0,Agilent6490|2|True|408.7581|17|Positive|b2|10|5|2|270.1925|380|50991.0,Agilent6490|3|True|408.7581|17|Positive|y4++|10|5|2|225.6386|380|35244.0,Agilent6490|4|True|408.7581|21|Positive|y4|10|5|2|450.2699|380|32887.0,Agilent6490|5|True|408.7581|17|Positive|y5++|10|5|2|274.165|380|26956.0,Agilent6490|1|False|403.7581|17|Positive|y5|10|5|2|537.3226|380|121071.0,Agilent6490|2|False|403.7581|17|Positive|b2|10|5|2|270.1925|380|50991.0,Agilent6490|3|False|403.7581|17|Positive|y4++|10|5|2|220.6386|380|35244.0,Agilent6490|4|False|403.7581|21|Positive|y4|10|5|2|440.2699|380|32887.0,Agilent6490|5|False|403.7581|17|Positive|y5++|10|5|2|269.165|380|26956.0 3.19(min) 3.19(min) 7.93 7.93 View Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4 6.712000 75.70
P53027 60S ribosomal protein L10a RPL10A Sus scrofa ILGPGLNK NA NA PEP2013062013

K+8

K+8 818.5 0.29

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|410.2631|5|Positive|b2|10|5|2|227.1754|380|593920.0,Agilent6490|2|True|410.2631|9|Positive|y6|10|5|2|593.3497|380|281489.0,Agilent6490|3|True|410.2631|9|Positive|y7|10|5|2|706.4338|380|59346.0,Agilent6490|4|True|410.2631|13|Positive|y5|10|5|2|536.3282|380|27607.0,Agilent6490|5|True|410.2631|17|Positive|y6++|10|5|2|297.1785|380|24835.0,Agilent6490|1|False|406.26|5|Positive|b2|10|5|2|227.1754|380|593920.0,Agilent6490|2|False|406.26|9|Positive|y6|10|5|2|585.33|380|281489.0,Agilent6490|3|False|406.26|9|Positive|y7|10|5|2|698.42|380|59346.0,Agilent6490|4|False|406.26|13|Positive|y5|10|5|2|528.31|380|27607.0,Agilent6490|5|False|406.26|17|Positive|y6++|10|5|2|293.17|380|24835.0 11.510(min) 11.510(min);11.50(min) 11.27 11.27;11.27 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 14.000000 100.00
Q29221 F-actin-capping protein subunit alpha-2 CAPZA2 Sus scrofa FTITPSTTQVVGILK NA NA PEP20120906011

K+8

K+8 1611.92 0.73

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|2|True|806.9742|33|Positive|b2|10|5|2|249.1239|380|1022.0,Agilent6490|1|True|806.9742|21|Positive|b4|10|5|2|463.2557|380|1124.0,Agilent6490|4|True|806.9742|49|Positive|y2|10|5|2|268.2116|380|800.0,Agilent6490|3|True|806.9742|29|Positive|y11++|10|5|2|575.8503|380|1005.0,Agilent6490|5|True|806.9742|25|Positive|y12++|10|5|2|626.3741|380|781.0,Agilent6490|2|False|802.9742|33|Positive|b2|10|5|2|249.1239|380|1022.0,Agilent6490|1|False|802.9742|21|Positive|b4|10|5|2|463.2557|380|1124.0,Agilent6490|4|False|802.9742|49|Positive|y2|10|5|2|260.2116|380|800.0,Agilent6490|3|False|802.9742|29|Positive|y11++|10|5|2|571.8503|380|1005.0,Agilent6490|5|False|802.9742|25|Positive|y12++|10|5|2|622.3741|380|781.0 34.310(min) 34.310(min);34.38(min) 27.22 27.22;27.47 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 12.260000 98.20
Q29238 Chloride intracellular channel protein 1 CLIC1 Sus scrofa GVTFNVTTVDTK NA NA PEP2013041732

K+8

K+8 1288.66 0.11

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|5|True|645.3455|21|Positive|b3|10|5|2|258.1448|380|1218.0,Agilent6490|4|True|645.3455|13|Positive|y3|10|5|2|371.2016|380|1263.0,Agilent6490|3|True|645.3455|29|Positive|y2|10|5|2|256.1747|380|1815.0,Agilent6490|2|True|645.3455|17|Positive|y8|10|5|2|885.4767|380|1820.0,Agilent6490|1|True|645.3455|17|Positive|y6|10|5|2|672.3654|380|2069.0,Agilent6490|5|False|641.3379|21|Positive|b3|10|5|2|258.15|380|1218.0,Agilent6490|4|False|641.3379|13|Positive|y3|10|5|2|363.19|380|1263.0,Agilent6490|3|False|641.3379|29|Positive|y2|10|5|2|248.16|380|1815.0,Agilent6490|2|False|641.3379|17|Positive|y8|10|5|2|877.46|380|1820.0,Agilent6490|1|False|641.3379|17|Positive|y6|10|5|2|664.35|380|2069.0 20.56(min) 20.56(min);20.630(min);20.40(min);20.395(min) 15.28 15.28;15.3;15.17;15.16 View Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 12,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.77|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 8,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.77|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 10.000000 100.00
Q767L6 Flotillin-1 FLOT1 Sus scrofa TEAEIAHIALETLEGHQR NA NA PEP2013110648

R+10

R+10 2027.02 -0.46

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|676.6849|21|Positive|y16++|10|5|3|899.4777|380|351028.0,Agilent6490|2|True|676.6849|25|Positive|b2|10|5|3|231.0975|380|248027.0,Agilent6490|3|True|676.6849|33|Positive|y4|10|5|3|507.2662|380|132233.0,Agilent6490|4|True|676.6849|25|Positive|y5|10|5|3|636.3088|380|103506.0,Agilent6490|5|True|676.6849|17|Positive|y16+++|10|5|3|599.9876|380|102233.0,Agilent6490|1|False|673.3516|21|Positive|y16++|10|5|3|894.4777|380|351028.0,Agilent6490|2|False|673.3516|25|Positive|b2|10|5|3|231.0975|380|248027.0,Agilent6490|3|False|673.3516|33|Positive|y4|10|5|3|497.2662|380|132233.0,Agilent6490|4|False|673.3516|25|Positive|y5|10|5|3|626.3088|380|103506.0,Agilent6490|5|False|673.3516|17|Positive|y16+++|10|5|3|596.6543|380|102233.0 31.920(min) 31.920(min) 21.87 21.87 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 NA 88.89
Q8SPS7 Haptoglobin HP Sus scrofa VGYVSGWGR NA NA PEP2013090505

R+10

R+10 989.49 -0.03

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|495.7557|9|Positive|y5|10|5|2|572.2815|380|24173.0,Agilent6490|2|True|495.7557|13|Positive|b3|10|5|2|320.1605|380|9565.0,Agilent6490|3|True|495.7557|13|Positive|y6|10|5|2|671.3499|380|7439.0,Agilent6490|4|True|495.7557|17|Positive|y8|10|5|2|891.4347|380|6686.0,Agilent6490|5|True|495.7557|13|Positive|y4|10|5|2|485.2495|380|6263.0,Agilent6490|1|False|490.75|9|Positive|y5|10|5|2|562.27|380|24173.0,Agilent6490|2|False|490.75|13|Positive|b3|10|5|2|320.1605|380|9565.0,Agilent6490|3|False|490.75|13|Positive|y6|10|5|2|661.34|380|7439.0,Agilent6490|4|False|490.75|17|Positive|y8|10|5|2|881.43|380|6686.0,Agilent6490|5|False|490.75|13|Positive|y4|10|5|2|475.24|380|6263.0 15.690(min) 15.690(min);16.827(min);16.27(min);16.27(min);9.95(min);16.827(min);16.6(min) 13.35 13.35;13.61;15.04;15.04;14.3;13.61;13.58 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 7,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 11,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 5,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|2.7|0.4, 2|9.9|0.4, 15|17.1|0.4, 22|26.1|0.4, 25|40.5|0.4, 27|81|0.4, 29|81|0.4, 30|2.7|0.4;Gradient 6,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 10,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|1.5|0.4, 1.50|6.3|0.4, 16.00|13.5|0.4, 18.00|13.77|0.4, 33.00|22.5|0.4, 38.00|40.5|0.4, 39.00|81|0.4, 42.90|81|0.4, 43.00|1.5|0.4 13.000000 47.70
P46405 40S ribosomal protein S12 RPS12 Sus scrofa TALIHDGLAR NA NA PEP2013062017

R+10

R+10 1075.59 0.34

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|359.5416|5|Positive|y8++|10|5|3|452.7656|380|95865.0,Agilent6490|2|True|359.5416|13|Positive|y6++|10|5|3|339.6815|380|88345.0,Agilent6490|3|True|359.5416|13|Positive|y5|10|5|3|541.2968|380|53821.0,Agilent6490|4|True|359.5416|5|Positive|y9++|10|5|3|488.2841|380|53397.0,Agilent6490|5|True|359.5416|5|Positive|y7++|10|5|3|396.2235|380|49726.0,Agilent6490|1|False|356.21|5|Positive|y8++|10|5|3|447.76|380|95865.0,Agilent6490|2|False|356.21|13|Positive|y6++|10|5|3|334.68|380|88345.0,Agilent6490|3|False|356.21|13|Positive|y5|10|5|3|531.29|380|53821.0,Agilent6490|4|False|356.21|5|Positive|y9++|10|5|3|483.28|380|53397.0,Agilent6490|5|False|356.21|5|Positive|y7++|10|5|3|391.22|380|49726.0 11.110(min) 11.110(min);11.11(min) 11.07 11.07;11.07 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 10.000000 100.00
Q28833 von Willebrand factor VWF Sus scrofa HIVTFDGQNFK NA NA PEP9999032403

K+8

K+8 1312.65 -0.40

Qtrap4000

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|438.563|5|Positive|b4|10|5|3|451.2669|380|513.0,Agilent6490|2|True|438.563|5|Positive|y5|10|5|3|601.3189|380|280.0,Agilent6490|3|True|438.563|9|Positive|b3|10|5|3|350.2192|380|263.0,Agilent6490|4|True|438.563|9|Positive|y6|10|5|3|716.3459|380|253.0,Agilent6490|5|True|438.563|5|Positive|y7++|10|5|3|432.2111|380|188.0,Agilent6490|1|False|435.896333333|5|Positive|b4|10|5|3|451.2669|380|513.0,Agilent6490|2|False|435.896333333|5|Positive|y5|10|5|3|593.3189|380|280.0,Agilent6490|3|False|435.896333333|9|Positive|b3|10|5|3|350.2192|380|263.0,Agilent6490|4|False|435.896333333|9|Positive|y6|10|5|3|708.3459|380|253.0,Agilent6490|5|False|435.896333333|5|Positive|y7++|10|5|3|428.2111|380|188.0;Instrument|Rank|Heavy|Q1|Q3|CollisionEnergy|DeclusteringPotential|FragmentIon|ParentChargeState|Time|CellExitPotential|Abundance,Qtrap4000|1|True|438.6|451.3|18|45|b4|3|20|21|0.0,Qtrap4000|2|True|438.6|716.3|17|45|y6|3|20|21|0.0,Qtrap4000|3|True|438.6|601.3|19|45|y5|3|20|21|0.0,Qtrap4000|4|True|438.6|432.2|13|45|y++7|3|20|21|0.0,Qtrap4000|5|True|438.6|358.7|15|45|y++6|3|20|21|0.0,Qtrap4000|1|False|435.9333|451.3|18|45|b4|3|20|21|0.0,Qtrap4000|2|False|435.9333|708.3|17|45|y6|3|20|21|0.0,Qtrap4000|3|False|435.9333|593.3|19|45|y5|3|20|21|0.0,Qtrap4000|4|False|435.9333|428.2|13|45|y++7|3|20|21|0.0,Qtrap4000|5|False|435.9333|354.7|15|45|y++6|3|20|21|0.0 16.460(min) 16.460(min);16.40(min);16.95(min) 13.56 13.56;13.56;15.14 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4 6.810000 98.10
P01315 Insulin INS Sus scrofa FVNQHLCGSHLVEALYLVCGER NA NA PEP9999041631

NA

NA 2486.22 0.44

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|625.0649|9|Positive|b16++|10|5|4|906.4485|380|118135.0,Agilent6490|2|True|625.0649|17|Positive|y4|10|5|4|474.2005|380|96567.0,Agilent6490|3|True|625.0649|13|Positive|b14++|10|5|4|768.3748|380|69952.0,Agilent6490|4|True|625.0649|9|Positive|b15++|10|5|4|824.9169|380|66486.0,Agilent6490|5|True|625.0649|9|Positive|y5|10|5|4|573.2689|380|63571.0,Agilent6490|1|False|622.5649|9|Positive|b16++|10|5|4|906.4485|380|118135.0,Agilent6490|2|False|622.5649|17|Positive|y4|10|5|4|464.2005|380|96567.0,Agilent6490|3|False|622.5649|13|Positive|b14++|10|5|4|768.3748|380|69952.0,Agilent6490|4|False|622.5649|9|Positive|b15++|10|5|4|824.9169|380|66486.0,Agilent6490|5|False|622.5649|9|Positive|y5|10|5|4|563.2689|380|63571.0 NA NA NA NA NA NA 8.450000 92.00
P26234 Vinculin VCL Sus scrofa ALASQLQDSLK NA NA PEP20130123046

K+8

K+8 1180.64 -0.09

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|591.335|21|Positive|y9|10|5|2|997.5404|380|527896.0,Agilent6490|2|True|591.335|17|Positive|y8|10|5|2|926.5033|380|416680.0,Agilent6490|3|True|591.335|13|Positive|y9++|10|5|2|499.2738|380|349294.0,Agilent6490|4|True|591.335|17|Positive|b3|10|5|2|256.1656|380|224785.0,Agilent6490|5|True|591.335|17|Positive|y5|10|5|2|598.3286|380|147788.0,Agilent6490|1|False|587.33|21|Positive|y9|10|5|2|989.53|380|527896.0,Agilent6490|2|False|587.33|17|Positive|y8|10|5|2|918.49|380|416680.0,Agilent6490|3|False|587.33|13|Positive|y9++|10|5|2|495.27|380|349294.0,Agilent6490|4|False|587.33|17|Positive|b3|10|5|2|256.1656|380|224785.0,Agilent6490|5|False|587.33|17|Positive|y5|10|5|2|590.31|380|147788.0 18.700(min) 18.700(min);18.70(min) 14.18 14.18;14.18 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 3,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|1.5|0.4, 1.50|6.3|0.4, 16.00|13.5|0.4, 18.00|13.77|0.4, 33.00|22.5|0.4, 38.00|40.5|0.4, 39.00|81|0.4, 42.90|81|0.4, 43.00|1.5|0.4 21.000000 98.00
P67937 Tropomyosin alpha-4 chain TPM4 Sus scrofa TIDDLEEK NA NA PEP20121106039

K+8

K+8 969.46 -1.29

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|485.7451|9|Positive|y6|10|5|2|756.3501|380|100641.0,Agilent6490|2|True|485.7451|9|Positive|b2|10|5|2|215.139|380|61060.0,Agilent6490|3|True|485.7451|21|Positive|y2|10|5|2|284.1696|380|13446.0,Agilent6490|4|True|485.7451|21|Positive|b6++|10|5|2|344.1634|380|12414.0,Agilent6490|5|True|485.7451|17|Positive|y4|10|5|2|526.2963|380|9885.0,Agilent6490|1|False|481.7375|9|Positive|y6|10|5|2|748.335|380|100641.0,Agilent6490|2|False|481.7375|9|Positive|b2|10|5|2|215.14|380|61060.0,Agilent6490|3|False|481.7375|21|Positive|y2|10|5|2|276.155|380|13446.0,Agilent6490|4|False|481.7375|21|Positive|b6++|10|5|2|344.16|380|12414.0,Agilent6490|5|False|481.7375|17|Positive|y4|10|5|2|518.281|380|9885.0 7.97(min) 7.97(min);7.740(min);7.69(min) 10.57 10.57;9.4;9.37 View Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 22.012000 100.00
P80895 Protein-L-isoaspartate(D-aspartate) O-methyltransferase PCMT1 Sus scrofa SGGASHSELIHNLR NA NA PEP2013061205

R+10

R+10 1486.74 -0.51

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|496.5917|21|Positive|y4|10|5|3|549.3131|380|23611.0,Agilent6490|2|True|496.5917|17|Positive|y4++|10|5|3|275.1602|380|19181.0,Agilent6490|3|True|496.5917|17|Positive|y3|10|5|3|412.2542|380|12369.0,Agilent6490|4|True|496.5917|13|Positive|y12+++|10|5|3|448.5733|380|10750.0,Agilent6490|5|True|496.5917|13|Positive|y13+++|10|5|3|467.5805|380|10431.0,Agilent6490|1|False|493.26|21|Positive|y4|10|5|3|539.3|380|23611.0,Agilent6490|2|False|493.26|17|Positive|y4++|10|5|3|270.16|380|19181.0,Agilent6490|3|False|493.26|17|Positive|y3|10|5|3|402.25|380|12369.0,Agilent6490|4|False|493.26|13|Positive|y12+++|10|5|3|445.24|380|10750.0,Agilent6490|5|False|493.26|13|Positive|y13+++|10|5|3|464.24|380|10431.0 8.03(min) 8.03(min);8.100(min) 9.54 9.54;9.58 View Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 7.200000 38.54
P20305 Gelsolin GSN Sus scrofa AGALNSNDAFVLK NA NA PEP9999032776

K+8

K+8 1326.69 0.34

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|664.359|21|Positive|b3|10|5|2|200.1035|380|1467.0,Agilent6490|3|True|664.359|13|Positive|b4|10|5|2|313.1876|380|352.0,Agilent6490|5|True|664.359|17|Positive|y4|10|5|2|514.3484|380|250.0,Agilent6490|2|True|664.359|29|Positive|y2|10|5|2|268.2116|380|649.0,Agilent6490|4|True|664.359|25|Positive|y3|10|5|2|367.28|380|325.0,Agilent6490|1|False|660.359|21|Positive|b3|10|5|2|200.1035|380|1467.0,Agilent6490|3|False|660.359|13|Positive|b4|10|5|2|313.1876|380|352.0,Agilent6490|5|False|660.359|17|Positive|y4|10|5|2|506.3484|380|250.0,Agilent6490|2|False|660.359|29|Positive|y2|10|5|2|260.2116|380|649.0,Agilent6490|4|False|660.359|25|Positive|y3|10|5|2|359.28|380|325.0 23.140(min) 23.140(min);23.82(min);23.14(min);22.878(min);23.82(min);22.88(min);24.01(min) 16.76 16.76;19.06;16.78;16.61;19.06;16.61;17.29 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 8,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.77|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 11,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 12,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.77|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 13,column: Agilent AdvanceBio Peptide Map 2.1 x 150mm 2.7um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 9.650000 97.30
O02773 Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA MAN1A1 Sus scrofa GGHSSSLFGNIK NA NA PEP2013101017

K+8

K+8 1210.6 -0.26

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|404.5474|9|Positive|y5|10|5|3|586.3439|380|492933.0,Agilent6490|2|True|404.5474|9|Positive|y4|10|5|3|439.2755|380|488142.0,Agilent6490|3|True|404.5474|5|Positive|y5++|10|5|3|293.6756|380|200584.0,Agilent6490|4|True|404.5474|5|Positive|b7|10|5|3|626.2893|380|160768.0,Agilent6490|5|True|404.5474|9|Positive|b6|10|5|3|513.2052|380|150056.0,Agilent6490|1|False|401.88|9|Positive|y5|10|5|3|578.33|380|492933.0,Agilent6490|2|False|401.88|9|Positive|y4|10|5|3|431.26|380|488142.0,Agilent6490|3|False|401.88|5|Positive|y5++|10|5|3|289.67|380|200584.0,Agilent6490|4|False|401.88|5|Positive|b7|10|5|3|626.2893|380|160768.0,Agilent6490|5|False|401.88|9|Positive|b6|10|5|3|513.2052|380|150056.0 14.100(min) 14.100(min) 12.56 12.56 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 NA 83.19
Q29092 Endoplasmin HSP90B1 Sus scrofa EEASDYLELDTIK NA NA PEP9999032646

K+8

K+8 1532.72 -0.79

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|767.3747|21|Positive|y4|10|5|2|484.2862|380|207.0,Agilent6490|2|True|767.3747|25|Positive|y6|10|5|2|726.4129|380|161.0,Agilent6490|3|True|767.3747|37|Positive|y3|10|5|2|369.2593|380|145.0,Agilent6490|4|True|767.3747|21|Positive|y5|10|5|2|597.3703|380|140.0,Agilent6490|5|True|767.3747|21|Positive|b3|10|5|2|330.1301|380|131.0,Agilent6490|1|False|763.3747|21|Positive|y4|10|5|2|476.2862|380|207.0,Agilent6490|2|False|763.3747|25|Positive|y6|10|5|2|718.4129|380|161.0,Agilent6490|3|False|763.3747|37|Positive|y3|10|5|2|361.2593|380|145.0,Agilent6490|4|False|763.3747|21|Positive|y5|10|5|2|589.3703|380|140.0,Agilent6490|5|False|763.3747|21|Positive|b3|10|5|2|330.1301|380|131.0 27.79(min) 27.79(min);27.11(min);27.080(min) 21.63 21.63;19.08;19.05 View Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 7.620000 91.70
P20305 Gelsolin GSN Sus scrofa TGAQELLR NA NA PEP20130129019

R+10

R+10 896.49 -0.40

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|449.2509|5|Positive|b3|10|5|2|230.1141|380|1466.0,Agilent6490|1|False|444.2509|5|Positive|b3|10|5|2|230.1141|380|1466.0,Agilent6490|4|True|449.2509|13|Positive|y3|10|5|2|411.3078|380|308.0,Agilent6490|4|False|444.2509|13|Positive|y3|10|5|2|401.3078|380|308.0,Agilent6490|2|True|449.2509|13|Positive|y4|10|5|2|540.3504|380|490.0,Agilent6490|2|False|444.2509|13|Positive|y4|10|5|2|530.3504|380|490.0,Agilent6490|3|True|449.2509|13|Positive|y5|10|5|2|668.4089|380|311.0,Agilent6490|3|False|444.2509|13|Positive|y5|10|5|2|658.4089|380|311.0,Agilent6490|5|True|449.2509|13|Positive|y6|10|5|2|739.446|380|204.0,Agilent6490|5|False|444.2509|13|Positive|y6|10|5|2|729.446|380|204.0 10.76(min) 10.76(min);10.76(min);11.822(min);6.44(min);10.53(min);11.822(min);10.550(min);11.7(min);11.29(min) 12.11 12.11;12.11;11.43;12.36;10.78;11.43;10.79;11.36;11.16 View Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 11,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 6,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 5,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|2.7|0.4, 2|9.9|0.4, 15|17.1|0.4, 22|26.1|0.4, 25|40.5|0.4, 27|81|0.4, 29|81|0.4, 30|2.7|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 7,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 10,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|1.5|0.4, 1.50|6.3|0.4, 16.00|13.5|0.4, 18.00|13.77|0.4, 33.00|22.5|0.4, 38.00|40.5|0.4, 39.00|81|0.4, 42.90|81|0.4, 43.00|1.5|0.4;Gradient 13,column: Agilent AdvanceBio Peptide Map 2.1 x 150mm 2.7um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 8.230000 100.00
P00503 Aspartate aminotransferase, cytoplasmic GOT1 Sus scrofa VGGVQSLGGTGALR NA NA PEP2013042521

R+10

R+10 1280.7 0.45

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|641.3616|25|Positive|y9|10|5|2|841.4766|380|84338.0,Agilent6490|2|True|641.3616|21|Positive|y10|10|5|2|969.5351|380|58427.0,Agilent6490|3|True|641.3616|21|Positive|y8|10|5|2|754.4445|380|43146.0,Agilent6490|4|True|641.3616|21|Positive|b4|10|5|2|313.187|380|27304.0,Agilent6490|5|True|641.3616|21|Positive|y6|10|5|2|584.339|380|19333.0,Agilent6490|1|False|636.36|25|Positive|y9|10|5|2|831.47|380|84338.0,Agilent6490|2|False|636.36|21|Positive|y10|10|5|2|959.53|380|58427.0,Agilent6490|3|False|636.36|21|Positive|y8|10|5|2|744.44|380|43146.0,Agilent6490|4|False|636.36|21|Positive|b4|10|5|2|313.187|380|27304.0,Agilent6490|5|False|636.36|21|Positive|y6|10|5|2|574.33|380|19333.0 16.410(min) 16.410(min) 13.56 13.56 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 8.090000 72.44
P24259 Natriuretic peptides A NPPA Sus scrofa DGGALGR NA NA PEP20120911033

R+10

R+10 654.32 -0.51

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|328.1741|5|Positive|b3|10|5|2|230.0777|380|1551.0,Agilent6490|3|True|328.1741|13|Positive|y2|10|5|2|242.1492|380|949.0,Agilent6490|2|True|328.1741|5|Positive|y3|10|5|2|355.2333|380|974.0,Agilent6490|5|True|328.1741|13|Positive|y4|10|5|2|426.2704|380|398.0,Agilent6490|4|True|328.1741|5|Positive|y5|10|5|2|483.2919|380|614.0,Agilent6490|1|False|323.1741|5|Positive|b3|10|5|2|230.0777|380|1551.0,Agilent6490|3|False|323.1741|13|Positive|y2|10|5|2|232.1492|380|949.0,Agilent6490|2|False|323.1741|5|Positive|y3|10|5|2|345.2333|380|974.0,Agilent6490|5|False|323.1741|13|Positive|y4|10|5|2|416.2704|380|398.0,Agilent6490|4|False|323.1741|5|Positive|y5|10|5|2|473.2919|380|614.0 3.08(min) 3.08(min);3.090(min);2.79(min) 7.08 7.08;7.09;7.71 View Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4 9.616000 69.50
P00336 L-lactate dehydrogenase B chain LDHB Sus scrofa GLTSVINQK NA NA PEP20130306025

K+8

K+8 966.54 -0.03

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|484.2873|9|Positive|y4|10|5|2|510.3126|380|8803.0,Agilent6490|2|True|484.2873|9|Positive|y7++|10|5|2|399.234|380|7025.0,Agilent6490|3|True|484.2873|13|Positive|y7|10|5|2|797.4607|380|5931.0,Agilent6490|4|True|484.2873|13|Positive|y6|10|5|2|696.413|380|4736.0,Agilent6490|5|True|484.2873|21|Positive|y3|10|5|2|397.2285|380|4579.0,Agilent6490|1|False|480.28|9|Positive|y4|10|5|2|502.3|380|8803.0,Agilent6490|2|False|480.28|9|Positive|y7++|10|5|2|395.23|380|7025.0,Agilent6490|3|False|480.28|13|Positive|y7|10|5|2|789.45|380|5931.0,Agilent6490|4|False|480.28|13|Positive|y6|10|5|2|688.4|380|4736.0,Agilent6490|5|False|480.28|21|Positive|y3|10|5|2|389.21|380|4579.0 12.780(min) 12.780(min);12.78(min);13.66(min) 11.9 11.9;11.9;12.34 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 13,column: Agilent AdvanceBio Peptide Map 2.1 x 150mm 2.7um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 8.000000 84.40
Q8SPS7 Haptoglobin HP Sus scrofa VGYVSGWGR NA NA PEP9999042405

R+10

R+10 989.49 -0.03

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|495.7557|13|Positive|y5|10|5|2|572.282|380|778.0,Agilent6490|2|True|495.7557|13|Positive|b3|10|5|2|320.161|380|511.0,Agilent6490|3|True|495.7557|17|Positive|y6|10|5|2|671.3505|380|286.0,Agilent6490|4|True|495.7557|9|Positive|y4|10|5|2|485.25|380|274.0,Agilent6490|5|True|495.7557|9|Positive|y7++|10|5|2|417.7108|380|256.0,Agilent6490|1|False|490.7557|13|Positive|y5|10|5|2|562.282|380|778.0,Agilent6490|2|False|490.7557|13|Positive|b3|10|5|2|320.161|380|511.0,Agilent6490|3|False|490.7557|17|Positive|y6|10|5|2|661.3505|380|286.0,Agilent6490|4|False|490.7557|9|Positive|y4|10|5|2|475.25|380|274.0,Agilent6490|5|False|490.7557|9|Positive|y7++|10|5|2|412.7108|380|256.0 NA NA NA NA NA NA 5.240000 NA
P63317 Troponin C, slow skeletal and cardiac muscles TNNC1 Sus scrofa AAVEQLTEEQK NA NA PEP20120911018

K+8

K+8 1252.62 -0.95

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|627.3274|5|Positive|b3|10|5|2|242.1505|380|1414.0,Agilent6490|4|True|627.3274|21|Positive|b4|10|5|2|371.1931|380|662.0,Agilent6490|5|True|627.3274|17|Positive|y4|10|5|2|541.2713|380|623.0,Agilent6490|3|True|627.3274|17|Positive|y5|10|5|2|642.319|380|691.0,Agilent6490|2|True|627.3274|17|Positive|y9++|10|5|2|556.2902|380|962.0,Agilent6490|1|False|623.3274|5|Positive|b3|10|5|2|242.1505|380|1414.0,Agilent6490|4|False|623.3274|21|Positive|b4|10|5|2|371.1931|380|662.0,Agilent6490|5|False|623.3274|17|Positive|y4|10|5|2|533.2713|380|623.0,Agilent6490|3|False|623.3274|17|Positive|y5|10|5|2|634.319|380|691.0,Agilent6490|2|False|623.3274|17|Positive|y9++|10|5|2|552.2902|380|962.0 8.86(min) 8.86(min);8.70(min);8.780(min) 11.06 11.06;9.88;9.91 View Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 10.081000 100.00
P68137 Actin, alpha skeletal muscle ACTA1 Sus scrofa GYSFVTTAER NA NA PEP9999032585

R+10

R+10 1139.54 -0.31

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|570.7822|5|Positive|b2|10|5|2|221.0926|380|2501.0,Agilent6490|4|True|570.7822|17|Positive|b3|10|5|2|308.1246|380|589.0,Agilent6490|2|True|570.7822|21|Positive|y5|10|5|2|587.3028|380|1117.0,Agilent6490|3|True|570.7822|13|Positive|y6|10|5|2|686.3713|380|797.0,Agilent6490|5|True|570.7822|5|Positive|y8++|10|5|2|460.7398|380|534.0,Agilent6490|1|False|565.7822|5|Positive|b2|10|5|2|221.0926|380|2501.0,Agilent6490|4|False|565.7822|17|Positive|b3|10|5|2|308.1246|380|589.0,Agilent6490|2|False|565.7822|21|Positive|y5|10|5|2|577.3028|380|1117.0,Agilent6490|3|False|565.7822|13|Positive|y6|10|5|2|676.3713|380|797.0,Agilent6490|5|False|565.7822|5|Positive|y8++|10|5|2|455.7398|380|534.0 15.410(min) 15.410(min);15.94(min);15.36(min) 13.21 13.21;14.97;13.18 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 6.210000 98.70
Q29116 Tenascin TNC Sus scrofa FTTDLDSPR NA NA PEP2016112422

R+10

R+10 1060.5 -0.97

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|531.2611|5|Positive|b2|10|5|2|249.1239|380|2517.0,Agilent6490|2|True|531.2611|25|Positive|y3|10|5|2|369.2126|380|639.0,Agilent6490|4|True|531.2611|17|Positive|y4|10|5|2|484.2395|380|347.0,Agilent6490|5|True|531.2611|29|Positive|y5|10|5|2|597.3236|380|285.0,Agilent6490|3|True|531.2611|17|Positive|y7|10|5|2|813.3982|380|408.0,Agilent6490|1|False|526.2611|5|Positive|b2|10|5|2|249.1239|380|2517.0,Agilent6490|2|False|526.2611|25|Positive|y3|10|5|2|359.2126|380|639.0,Agilent6490|4|False|526.2611|17|Positive|y4|10|5|2|474.2395|380|347.0,Agilent6490|5|False|526.2611|29|Positive|y5|10|5|2|587.3236|380|285.0,Agilent6490|3|False|526.2611|17|Positive|y7|10|5|2|803.3982|380|408.0 NA NA NA NA NA NA 7.000000 98.42
P01025 Complement C3 C3 Sus scrofa TGLQEVEVK NA NA PEP20130129010

K+8

K+8 1009.54 -0.37

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|504.7876|17|Positive|y6|10|5|2|737.4141|380|1047.0,Agilent6490|2|True|504.7876|13|Positive|y7++|10|5|2|425.753|380|943.0,Agilent6490|3|True|504.7876|17|Positive|y5|10|5|2|609.3555|380|779.0,Agilent6490|4|True|504.7876|13|Positive|b3|10|5|2|272.161|380|663.0,Agilent6490|5|True|504.7876|17|Positive|y4|10|5|2|480.3129|380|428.0,Agilent6490|1|False|501.7876|17|Positive|y6|10|5|2|731.4141|380|1047.0,Agilent6490|2|False|501.7876|13|Positive|y7++|10|5|2|422.753|380|943.0,Agilent6490|3|False|501.7876|17|Positive|y5|10|5|2|603.3555|380|779.0,Agilent6490|4|False|501.7876|13|Positive|b3|10|5|2|272.161|380|663.0,Agilent6490|5|False|501.7876|17|Positive|y4|10|5|2|474.3129|380|428.0 12.6(min) 12.6(min);11.92(min);12.28(min);11.59(min);11.590(min);12.786(min);11.55(min);12.786(min);6.83(min);11.92(min) 11.81 11.81;12.75;11.65;11.31;11.31;11.9;11.29;11.9;12.58;12.75 View Gradient 10,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|1.5|0.4, 1.50|6.3|0.4, 16.00|13.5|0.4, 18.00|13.77|0.4, 33.00|22.5|0.4, 38.00|40.5|0.4, 39.00|81|0.4, 42.90|81|0.4, 43.00|1.5|0.4;Gradient 11,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 13,column: Agilent AdvanceBio Peptide Map 2.1 x 150mm 2.7um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 3,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|1.5|0.4, 1.50|6.3|0.4, 16.00|13.5|0.4, 18.00|13.77|0.4, 33.00|22.5|0.4, 38.00|40.5|0.4, 39.00|81|0.4, 42.90|81|0.4, 43.00|1.5|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 7,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 6,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 5,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|2.7|0.4, 2|9.9|0.4, 15|17.1|0.4, 22|26.1|0.4, 25|40.5|0.4, 27|81|0.4, 29|81|0.4, 30|2.7|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4 7.950000 91.50
Q29074 Beta-nerve growth factor NGF Sus scrofa LQHSLDTALR NA NA PEP9999032597

R+10

R+10 1162.63 -0.30

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|582.3245|17|Positive|y8++|10|5|2|461.7527|380|464530.0,Agilent6490|2|True|582.3245|25|Positive|y7|10|5|2|785.4391|380|236787.0,Agilent6490|3|True|582.3245|21|Positive|y8|10|5|2|922.498|380|191323.0,Agilent6490|4|True|582.3245|25|Positive|y4|10|5|2|470.2961|380|128309.0,Agilent6490|5|True|582.3245|21|Positive|b3|10|5|2|379.2088|380|89848.0,Agilent6490|1|False|577.32|17|Positive|y8++|10|5|2|456.75|380|464530.0,Agilent6490|2|False|577.32|25|Positive|y7|10|5|2|775.43|380|236787.0,Agilent6490|3|False|577.32|21|Positive|y8|10|5|2|912.49|380|191323.0,Agilent6490|4|False|577.32|25|Positive|y4|10|5|2|460.29|380|128309.0,Agilent6490|5|False|577.32|21|Positive|b3|10|5|2|379.2088|380|89848.0 10.33(min) 10.33(min);10.370(min);10.74(min) 10.68 10.68;10.7;12.1 View Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4 6.640000 98.90
Q9TSX9 Peroxiredoxin-6 PRDX6 Sus scrofa FHDFLGDSWGILFSHPR NA NA PEP2013040948

R+10

R+10 2039.98 -0.14

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|511.0049|9|Positive|y8++|10|5|4|468.7681|380|30296.0,Agilent6490|2|True|511.0049|9|Positive|y4|10|5|4|506.2709|380|15313.0,Agilent6490|3|True|511.0049|9|Positive|y9++|10|5|4|561.8078|380|9365.0,Agilent6490|4|True|511.0049|25|Positive|b3|10|5|4|400.1615|380|7111.0,Agilent6490|5|True|511.0049|21|Positive|y5|10|5|4|653.3393|380|6192.0,Agilent6490|1|False|508.5|9|Positive|y8++|10|5|4|463.76|380|30296.0,Agilent6490|2|False|508.5|9|Positive|y4|10|5|4|496.26|380|15313.0,Agilent6490|3|False|508.5|9|Positive|y9++|10|5|4|556.8|380|9365.0,Agilent6490|4|False|508.5|25|Positive|b3|10|5|4|400.1615|380|7111.0,Agilent6490|5|False|508.5|21|Positive|y5|10|5|4|643.33|380|6192.0 36.200(min) 36.200(min);36.138(min);36.14(min) 34.02 34.02;33.8;33.8 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 8,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.77|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 12,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.77|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 NA 80.83
Q9GLP1 Coagulation factor V F5 Sus scrofa AEVDDVIQVR NA NA PEP20130217023

R+10

R+10 1152.59 0.04

Qtrap4000

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|5|True|577.3085|13|Positive|b3|10|5|2|300.1559|380|276.0,Agilent6490|4|True|577.3085|13|Positive|y3|10|5|2|412.2548|380|285.0,Agilent6490|2|True|577.3085|13|Positive|y8++|10|5|2|477.2687|380|419.0,Agilent6490|1|True|577.3085|17|Positive|y4|10|5|2|525.3388|380|437.0,Agilent6490|3|True|577.3085|17|Positive|y7|10|5|2|854.4611|380|328.0,Agilent6490|5|False|572.3085|13|Positive|b3|10|5|2|300.1559|380|276.0,Agilent6490|4|False|572.3085|13|Positive|y3|10|5|2|402.2548|380|285.0,Agilent6490|2|False|572.3085|13|Positive|y8++|10|5|2|472.2687|380|419.0,Agilent6490|1|False|572.3085|17|Positive|y4|10|5|2|515.3388|380|437.0,Agilent6490|3|False|572.3085|17|Positive|y7|10|5|2|844.4611|380|328.0;Instrument|Rank|Heavy|Q1|Q3|CollisionEnergy|DeclusteringPotential|FragmentIon|ParentChargeState|Time|CellExitPotential|Abundance,Qtrap4000|1|True|577.3|854.5|29|69|y7|2|20|21|0.0,Qtrap4000|2|True|577.3|953.5|26|69|y8|2|20|21|0.0,Qtrap4000|3|True|577.3|525.3|29|69|y4|2|20|21|0.0,Qtrap4000|4|True|577.3|739.4|34|69|y6|2|20|21|0.0,Qtrap4000|5|True|577.3|300.2|26|69|b3|2|20|21|0.0,Qtrap4000|1|False|572.3|844.5|29|69|y7|2|20|21|0.0,Qtrap4000|2|False|572.3|943.5|26|69|y8|2|20|21|0.0,Qtrap4000|3|False|572.3|515.3|29|69|y4|2|20|21|0.0,Qtrap4000|4|False|572.3|729.4|34|69|y6|2|20|21|0.0,Qtrap4000|5|False|572.3|300.2|26|69|b3|2|20|21|0.0 17.753(min) 17.753(min);17.810(min);18.45(min);17.80(min);18.45(min) 13.76 13.76;13.74;15.59;13.77;15.59 View Gradient 9,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|2.7|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.8|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 11,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4 6.070000 98.70
P68137 Actin, alpha skeletal muscle ACTA1 Sus scrofa VAPEEHPTLLTEAPLNPK NA NA PEP20120911086

K+8

K+8 1963.04 -0.54

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|4|True|655.358|49|Positive|y2|10|5|3|252.1803|380|163.0,Agilent6490|3|True|655.358|9|Positive|y5|10|5|3|576.3601|380|163.0,Agilent6490|5|True|655.358|21|Positive|y6|10|5|3|647.3972|380|144.0,Agilent6490|2|True|655.358|17|Positive|y16++|10|5|3|897.4804|380|257.0,Agilent6490|1|True|655.358|13|Positive|y16+++|10|5|3|598.6562|380|659.0,Agilent6490|4|False|652.6913|49|Positive|y2|10|5|3|244.1803|380|163.0,Agilent6490|3|False|652.6913|9|Positive|y5|10|5|3|568.3601|380|163.0,Agilent6490|5|False|652.6913|21|Positive|y6|10|5|3|639.3972|380|144.0,Agilent6490|2|False|652.6913|17|Positive|y16++|10|5|3|893.4804|380|257.0,Agilent6490|1|False|652.6913|13|Positive|y16+++|10|5|3|595.9895|380|659.0 NA NA NA NA NA NA 8.151000 78.90
P00503 Aspartate aminotransferase, cytoplasmic GOT1 Sus scrofa NLDYVATSIHEAVTK NA NA PEP2013030607

K+8

K+8 1667.85 -0.05

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|556.9615|13|Positive|y13++|10|5|3|721.3743|380|13337.0,Agilent6490|2|True|556.9615|13|Positive|b2|10|5|3|228.1343|380|4626.0,Agilent6490|3|True|556.9615|17|Positive|y10++|10|5|3|532.7949|380|2862.0,Agilent6490|4|True|556.9615|9|Positive|y12++|10|5|3|663.8608|380|2402.0,Agilent6490|5|True|556.9615|17|Positive|y11++|10|5|3|582.3291|380|2044.0,Agilent6490|1|False|554.29|13|Positive|y13++|10|5|3|717.37|380|13337.0,Agilent6490|2|False|554.29|13|Positive|b2|10|5|3|228.1343|380|4626.0,Agilent6490|3|False|554.29|17|Positive|y10++|10|5|3|528.79|380|2862.0,Agilent6490|4|False|554.29|9|Positive|y12++|10|5|3|659.85|380|2402.0,Agilent6490|5|False|554.29|17|Positive|y11++|10|5|3|578.32|380|2044.0 25.380(min) 25.380(min);25.31(min) 18.07 18.07;18.04 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 3.000000 100.00
P18650 Apolipoprotein E APOE Sus scrofa LGPLVEQGR NA NA PEP2013013103

R+10

R+10 977.55 -0.23

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|3|True|489.78|17|Positive|y4|10|5|2|499.2504|380|1682.0,Agilent6490|3|False|484.78|17|Positive|y4|10|5|2|489.2504|380|1682.0,Agilent6490|2|True|489.78|21|Positive|y5|10|5|2|598.3188|380|1905.0,Agilent6490|2|False|484.78|21|Positive|y5|10|5|2|588.3188|380|1905.0,Agilent6490|5|True|489.78|21|Positive|y6|10|5|2|711.4029|380|932.0,Agilent6490|5|False|484.78|21|Positive|y6|10|5|2|701.4029|380|932.0,Agilent6490|1|True|489.78|13|Positive|y7++|10|5|2|404.7317|380|7835.0,Agilent6490|1|False|484.78|13|Positive|y7++|10|5|2|399.7317|380|7835.0,Agilent6490|4|True|489.78|13|Positive|y8++|10|5|2|433.2425|380|1496.0,Agilent6490|4|False|484.78|13|Positive|y8++|10|5|2|428.2425|380|1496.0 12.350(min) 12.350(min);12.67(min);7.52(min);13.642(min);13.642(min);12.34(min);12.67(min);13.5(min);12.89(min) 11.69 11.69;13.16;12.96;12.33;12.33;11.68;13.16;12.26;11.96 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 5,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|2.7|0.4, 2|9.9|0.4, 15|17.1|0.4, 22|26.1|0.4, 25|40.5|0.4, 27|81|0.4, 29|81|0.4, 30|2.7|0.4;Gradient 6,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 7,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 11,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 10,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|1.5|0.4, 1.50|6.3|0.4, 16.00|13.5|0.4, 18.00|13.77|0.4, 33.00|22.5|0.4, 38.00|40.5|0.4, 39.00|81|0.4, 42.90|81|0.4, 43.00|1.5|0.4;Gradient 13,column: Agilent AdvanceBio Peptide Map 2.1 x 150mm 2.7um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 6.130000 96.00
P20305 Gelsolin GSN Sus scrofa AGALNSNDAFVLK NA NA PEP2013080727

K+8

K+8 1326.69 0.34

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|664.359|21|Positive|b3|10|5|2|200.1035|380|1467.0,Agilent6490|3|True|664.359|13|Positive|b4|10|5|2|313.1876|380|352.0,Agilent6490|5|True|664.359|17|Positive|y4|10|5|2|514.3484|380|250.0,Agilent6490|2|True|664.359|29|Positive|y2|10|5|2|268.2116|380|649.0,Agilent6490|4|True|664.359|25|Positive|y3|10|5|2|367.28|380|325.0,Agilent6490|1|False|660.359|21|Positive|b3|10|5|2|200.1035|380|1467.0,Agilent6490|3|False|660.359|13|Positive|b4|10|5|2|313.1876|380|352.0,Agilent6490|5|False|660.359|17|Positive|y4|10|5|2|506.3484|380|250.0,Agilent6490|2|False|660.359|29|Positive|y2|10|5|2|260.2116|380|649.0,Agilent6490|4|False|660.359|25|Positive|y3|10|5|2|359.28|380|325.0 24.541(min) 24.541(min);24.541(min);24.2(min) 17.58 17.58;17.58;17.38 View Gradient 6,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 7,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 10,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|1.5|0.4, 1.50|6.3|0.4, 16.00|13.5|0.4, 18.00|13.77|0.4, 33.00|22.5|0.4, 38.00|40.5|0.4, 39.00|81|0.4, 42.90|81|0.4, 43.00|1.5|0.4 9.000000 97.30
P53027 60S ribosomal protein L10a RPL10A Sus scrofa DTLYEAVR NA NA PEP2013062015

R+10

R+10 975.48 -0.46

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|488.7529|5|Positive|b2|10|5|2|217.0819|380|74803.0,Agilent6490|2|True|488.7529|9|Positive|y5|10|5|2|647.3387|380|50265.0,Agilent6490|3|True|488.7529|9|Positive|y6|10|5|2|760.4227|380|24409.0,Agilent6490|4|True|488.7529|9|Positive|y4|10|5|2|484.2753|380|23185.0,Agilent6490|5|True|488.7529|17|Positive|y3|10|5|2|355.2328|380|13166.0,Agilent6490|1|False|483.75|5|Positive|b2|10|5|2|217.0819|380|74803.0,Agilent6490|2|False|483.75|9|Positive|y5|10|5|2|637.33|380|50265.0,Agilent6490|3|False|483.75|9|Positive|y6|10|5|2|750.41|380|24409.0,Agilent6490|4|False|483.75|9|Positive|y4|10|5|2|474.27|380|23185.0,Agilent6490|5|False|483.75|17|Positive|y3|10|5|2|345.22|380|13166.0 13.280(min) 13.280(min);13.25(min) 12.15 12.15;12.13 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 12.000000 100.00
O77666 Tripartite motif-containing protein 26 TRIM26 Sus scrofa ILNHLSTLR NA NA PEP20130131028

R+10

R+10 1075.63 0.36

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|5|True|359.5537|12|Positive|y4|10|5|3|486.291|380|42436.0,Agilent6490|4|True|359.5537|9|Positive|y5|10|5|3|599.3751|380|43652.0,Agilent6490|3|True|359.5537|8|Positive|y6++|10|5|3|368.7206|380|47567.0,Agilent6490|2|True|359.5537|4|Positive|y7++|10|5|3|425.7421|380|81861.0,Agilent6490|1|True|359.5537|4|Positive|y8++|10|5|3|482.2841|380|120515.0,Agilent6490|5|False|356.2171|12|Positive|y4|10|5|3|476.28|380|42436.0,Agilent6490|4|False|356.2171|9|Positive|y5|10|5|3|589.37|380|43652.0,Agilent6490|3|False|356.2171|8|Positive|y6++|10|5|3|363.72|380|47567.0,Agilent6490|2|False|356.2171|4|Positive|y7++|10|5|3|420.74|380|81861.0,Agilent6490|1|False|356.2171|4|Positive|y8++|10|5|3|477.28|380|120515.0 13.36(min) 13.36(min);12.900(min);12.86(min) 13.54 13.54;11.96;11.94 View Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 10.974000 100.00
P01315 Insulin INS Sus scrofa GFFYTPK NA NA PEP9999032774

K+8

K+8 866.43 -0.33

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|4|True|434.2287|5|Positive|b3|10|5|2|352.1661|380|451.0,Agilent6490|3|True|434.2287|17|Positive|y3|10|5|2|353.228|380|746.0,Agilent6490|2|True|434.2287|9|Positive|y4|10|5|2|516.2913|380|848.0,Agilent6490|1|True|434.2287|9|Positive|y5|10|5|2|663.3597|380|2224.0,Agilent6490|5|True|434.2287|9|Positive|y6++|10|5|2|405.718|380|314.0,Agilent6490|4|False|430.2287|5|Positive|b3|10|5|2|352.1661|380|451.0,Agilent6490|3|False|430.2287|17|Positive|y3|10|5|2|345.228|380|746.0,Agilent6490|2|False|430.2287|9|Positive|y4|10|5|2|508.2913|380|848.0,Agilent6490|1|False|430.2287|9|Positive|y5|10|5|2|655.3597|380|2224.0,Agilent6490|5|False|430.2287|9|Positive|y6++|10|5|2|401.718|380|314.0 19.850(min) 19.850(min);20.69(min);19.82(min) 14.85 14.85;17.04;14.86 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 8.200000 97.40
P62802 Histone H4 NA Sus scrofa DNIQGITKPAIR NA NA PEP2013041730

R+10

R+10 1334.75 -0.53

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|5|True|445.926|9|Positive|y9++|10|5|3|497.3076|380|4163.0,Agilent6490|4|True|445.926|13|Positive|y4|10|5|3|466.3012|380|8726.0,Agilent6490|3|True|445.926|9|Positive|y8++|10|5|3|433.2783|380|10169.0,Agilent6490|2|True|445.926|9|Positive|y10++|10|5|3|553.8496|380|10843.0,Agilent6490|1|True|445.926|5|Positive|b2|10|5|3|230.0771|380|32892.0,Agilent6490|5|False|442.589|9|Positive|y9++|10|5|3|492.3|380|4163.0,Agilent6490|4|False|442.589|13|Positive|y4|10|5|3|456.29|380|8726.0,Agilent6490|3|False|442.589|9|Positive|y8++|10|5|3|428.27|380|10169.0,Agilent6490|2|False|442.589|9|Positive|y10++|10|5|3|548.85|380|10843.0,Agilent6490|1|False|442.589|5|Positive|b2|10|5|3|230.077|380|32892.0 13.453(min) 13.453(min);13.45(min) 12.24 12.24;12.23 View Gradient 8,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.77|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 12,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.77|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 9.000000 100.00
P80272 Non-histone chromosomal protein HMG-17 HMGN2 Sus scrofa AEGAGDAK NA NA PEP2013102817

K+8

K+8 725.33 -0.79

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|363.6796|5|Positive|b2|10|5|2|201.087|380|3157.0,Agilent6490|2|True|363.6796|9|Positive|y6|10|5|2|526.2711|380|2917.0,Agilent6490|3|True|363.6796|13|Positive|y2|10|5|2|226.1641|380|1697.0,Agilent6490|4|True|363.6796|13|Positive|y4|10|5|2|398.2125|380|581.0,Agilent6490|5|True|363.6796|9|Positive|y5|10|5|2|469.2496|380|526.0,Agilent6490|1|False|359.67|5|Positive|b2|10|5|2|201.087|380|3157.0,Agilent6490|2|False|359.67|9|Positive|y6|10|5|2|518.26|380|2917.0,Agilent6490|3|False|359.67|13|Positive|y2|10|5|2|218.15|380|1697.0,Agilent6490|4|False|359.67|13|Positive|y4|10|5|2|390.2|380|581.0,Agilent6490|5|False|359.67|9|Positive|y5|10|5|2|461.23|380|526.0 1.310(min) 1.310(min) 5.69 5.69 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 5.000000 78.71
O19113 CCN family member 2 CCN2 Sus scrofa TTTLPVEFK NA NA PEP2013040232

K+8

K+8 1042.56 -0.03

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|5|True|522.2973|13|Positive|y6|10|5|2|740.4433|380|2075.0,Agilent6490|4|True|522.2973|13|Positive|y2|10|5|2|302.1954|380|2452.0,Agilent6490|3|True|522.2973|13|Positive|y5|10|5|2|627.3592|380|7136.0,Agilent6490|2|True|522.2973|9|Positive|y7|10|5|2|841.4909|380|7830.0,Agilent6490|1|True|522.2973|5|Positive|b2|10|5|2|203.1026|380|33708.0,Agilent6490|5|False|518.2897|13|Positive|y6|10|5|2|732.43|380|2075.0,Agilent6490|4|False|518.2897|13|Positive|y2|10|5|2|294.18|380|2452.0,Agilent6490|3|False|518.2897|13|Positive|y5|10|5|2|619.34|380|7136.0,Agilent6490|2|False|518.2897|9|Positive|y7|10|5|2|833.48|380|7830.0,Agilent6490|1|False|518.2897|5|Positive|b2|10|5|2|203.1026|380|33708.0 22.090(min) 22.090(min);22.09(min) 16.15 16.15;16.17 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 8.000000 100.00
P80895 Protein-L-isoaspartate(D-aspartate) O-methyltransferase PCMT1 Sus scrofa VQLVVGDGR NA NA PEP2013110620

R+10

R+10 951.53 0.46

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|476.7767|13|Positive|b2|10|5|2|228.1343|380|1151506.0,Agilent6490|2|True|476.7767|13|Positive|y7|10|5|2|725.418|380|1037841.0,Agilent6490|3|True|476.7767|21|Positive|y2|10|5|2|242.1487|380|775421.0,Agilent6490|4|True|476.7767|13|Positive|y5|10|5|2|513.2655|380|553718.0,Agilent6490|5|True|476.7767|13|Positive|y6|10|5|2|612.3339|380|464241.0,Agilent6490|1|False|471.77|13|Positive|b2|10|5|2|228.1343|380|1151506.0,Agilent6490|2|False|471.77|13|Positive|y7|10|5|2|715.41|380|1037841.0,Agilent6490|3|False|471.77|21|Positive|y2|10|5|2|232.14|380|775421.0,Agilent6490|4|False|471.77|13|Positive|y5|10|5|2|503.26|380|553718.0,Agilent6490|5|False|471.77|13|Positive|y6|10|5|2|602.33|380|464241.0 12.510(min) 12.510(min) 11.77 11.77 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 13.700000 91.55
P12263 Coagulation factor VIII F8 Sus scrofa LHPTHYSIR NA NA PEP2013112627

R+10

R+10 1132.59 -0.78

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|378.5417|9|Positive|y7++|10|5|3|442.2366|380|34638.0,Agilent6490|2|True|378.5417|13|Positive|y4|10|5|3|548.3066|380|16956.0,Agilent6490|3|True|378.5417|9|Positive|y8+++|10|5|3|340.8465|380|15043.0,Agilent6490|4|True|378.5417|9|Positive|y5++|10|5|3|343.1864|380|14737.0,Agilent6490|5|True|378.5417|13|Positive|y3|10|5|3|385.2433|380|8038.0,Agilent6490|1|False|375.21|9|Positive|y7++|10|5|3|437.23|380|34638.0,Agilent6490|2|False|375.21|13|Positive|y4|10|5|3|538.3|380|16956.0,Agilent6490|3|False|375.21|9|Positive|y8+++|10|5|3|337.51|380|15043.0,Agilent6490|4|False|375.21|9|Positive|y5++|10|5|3|338.18|380|14737.0,Agilent6490|5|False|375.21|13|Positive|y3|10|5|3|375.23|380|8038.0 5.02(min) 5.02(min);5.080(min) 8.05 8.05;8.08 View Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 7.000000 100.00
P81558 Myelin basic protein MBP Sus scrofa HGFLPR NA NA PEP2013040213

R+10

R+10 735.4 -0.52

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|368.7106|9|Positive|y5|10|5|2|599.3539|380|3999.0,Agilent6490|2|True|368.7106|9|Positive|y4|10|5|2|542.3325|380|1984.0,Agilent6490|3|True|368.7106|13|Positive|y2|10|5|2|282.18|380|1064.0,Agilent6490|4|True|368.7106|21|Positive|b3|10|5|2|342.1561|380|621.0,Agilent6490|5|True|368.7106|13|Positive|b4|10|5|2|455.2401|380|411.0,Agilent6490|1|False|363.706|9|Positive|y5|10|5|2|589.35|380|3999.0,Agilent6490|2|False|363.706|9|Positive|y4|10|5|2|532.32|380|1984.0,Agilent6490|3|False|363.706|13|Positive|y2|10|5|2|272.17|380|1064.0,Agilent6490|4|False|363.706|21|Positive|b3|10|5|2|342.1561|380|621.0,Agilent6490|5|False|363.706|13|Positive|b4|10|5|2|455.2401|380|411.0 7.85(min) 7.85(min);7.870(min) 9.45 9.45;9.46 View Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 7.000000 100.00
P26042 Moesin MSN Sus scrofa IQVWHEEHR NA NA PEP20130311028

R+10

R+10 1242.61 -1.51

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|415.2123|9|Positive|y7++|10|5|3|501.7426|380|98028.0,Agilent6490|2|True|415.2123|13|Positive|y5++|10|5|3|359.1688|380|83491.0,Agilent6490|3|True|415.2123|5|Positive|y8++|10|5|3|565.7719|380|55399.0,Agilent6490|4|True|415.2123|17|Positive|y5|10|5|3|717.3302|380|39551.0,Agilent6490|5|True|415.2123|17|Positive|y2|10|5|3|322.1861|380|37620.0,Agilent6490|1|False|411.876|9|Positive|y7++|10|5|3|496.74|380|98028.0,Agilent6490|2|False|411.876|13|Positive|y5++|10|5|3|354.16|380|83491.0,Agilent6490|3|False|411.876|5|Positive|y8++|10|5|3|560.77|380|55399.0,Agilent6490|4|False|411.876|17|Positive|y5|10|5|3|707.32|380|39551.0,Agilent6490|5|False|411.876|17|Positive|y2|10|5|3|312.18|380|37620.0 6.740(min) 6.740(min) 8.9 8.9 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 8.000000 100.00
P01025 Complement C3 C3 Sus scrofa TIYTPGSTVLYR NA NA PEP9999032714

R+10

R+10 1379.72 0.04

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|690.874|17|Positive|b2|10|5|2|215.139|380|12683.0,Agilent6490|3|True|690.874|21|Positive|y8++|10|5|2|451.7521|380|7114.0,Agilent6490|5|True|690.874|13|Positive|b4|10|5|2|479.25|380|4753.0,Agilent6490|4|True|690.874|13|Positive|y10++|10|5|2|583.8076|380|5910.0,Agilent6490|2|True|690.874|21|Positive|y8|10|5|2|902.497|380|10925.0,Agilent6490|1|False|685.874|17|Positive|b2|10|5|2|215.139|380|12683.0,Agilent6490|3|False|685.874|21|Positive|y8++|10|5|2|446.7521|380|7114.0,Agilent6490|5|False|685.874|13|Positive|b4|10|5|2|479.25|380|4753.0,Agilent6490|4|False|685.874|13|Positive|y10++|10|5|2|578.8076|380|5910.0,Agilent6490|2|False|685.874|21|Positive|y8|10|5|2|892.497|380|10925.0 23.96(min) 23.96(min);23.96(min);23.37(min);23.36(min);23.360(min) 19.15 19.15;19.15;16.91;16.89;16.89 View Gradient 11,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 3,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|1.5|0.4, 1.50|6.3|0.4, 16.00|13.5|0.4, 18.00|13.77|0.4, 33.00|22.5|0.4, 38.00|40.5|0.4, 39.00|81|0.4, 42.90|81|0.4, 43.00|1.5|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 8.860000 79.70
P20112 SPARC SPARC Sus scrofa NVLVTLYER NA NA PEP20120911039

R+10

R+10 1115.61 0.28

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|558.8185|5|Positive|b2|10|5|2|214.1192|380|5999.0,Agilent6490|2|True|558.8185|13|Positive|b3|10|5|2|327.2032|380|1054.0,Agilent6490|4|True|558.8185|13|Positive|y5|10|5|2|691.3654|380|681.0,Agilent6490|3|True|558.8185|9|Positive|y6|10|5|2|790.4339|380|880.0,Agilent6490|5|True|558.8185|13|Positive|y7|10|5|2|903.5179|380|616.0,Agilent6490|1|False|553.8185|5|Positive|b2|10|5|2|214.1192|380|5999.0,Agilent6490|2|False|553.8185|13|Positive|b3|10|5|2|327.2032|380|1054.0,Agilent6490|4|False|553.8185|13|Positive|y5|10|5|2|681.3654|380|681.0,Agilent6490|3|False|553.8185|9|Positive|y6|10|5|2|780.4339|380|880.0,Agilent6490|5|False|553.8185|13|Positive|y7|10|5|2|893.5179|380|616.0 25.27(min) 25.27(min);24.640(min);24.57(min) 20 20;17.63;17.61 View Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 6.484000 67.00
O02668 Inter-alpha-trypsin inhibitor heavy chain H2 ITIH2 Sus scrofa VQFELHYQEVK NA NA PEP9999032710

K+8

K+8 1426.72 -0.67

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|476.5857|5|Positive|b2|10|5|3|228.1348|380|5477.0,Agilent6490|4|True|476.5857|17|Positive|y2|10|5|3|254.196|380|549.0,Agilent6490|5|True|476.5857|13|Positive|y7++|10|5|3|462.7556|380|481.0,Agilent6490|2|True|476.5857|9|Positive|y9++|10|5|3|600.8111|380|2329.0,Agilent6490|3|True|476.5857|9|Positive|y10++|10|5|3|664.8404|380|804.0,Agilent6490|1|False|473.919|5|Positive|b2|10|5|3|228.1348|380|5477.0,Agilent6490|4|False|473.919|17|Positive|y2|10|5|3|246.196|380|549.0,Agilent6490|5|False|473.919|13|Positive|y7++|10|5|3|458.7556|380|481.0,Agilent6490|2|False|473.919|9|Positive|y9++|10|5|3|596.8111|380|2329.0,Agilent6490|3|False|473.919|9|Positive|y10++|10|5|3|660.8404|380|804.0 20.81(min) 20.81(min);19.758(min);20.81(min);20.050(min);19.98(min);19.76(min) 17.12 17.12;14.79;17.12;14.96;14.95;14.79 View Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 8,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.77|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 11,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 12,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.77|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 7.590000 99.00
Q08092 Calponin-1 CNN1 Sus scrofa GPAYGLSAEVK NA NA PEP2014012817

K+8

K+8 1098.57 -0.03

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|550.2979|21|Positive|y7|10|5|2|711.4127|380|78851.0,Agilent6490|2|True|550.2979|17|Positive|b3|10|5|2|226.1186|380|68812.0,Agilent6490|3|True|550.2979|17|Positive|y8|10|5|2|874.476|380|65056.0,Agilent6490|4|True|550.2979|17|Positive|y9|10|5|2|945.5131|380|43579.0,Agilent6490|5|True|550.2979|17|Positive|y5|10|5|2|541.3072|380|43313.0,Agilent6490|1|False|546.29|21|Positive|y7|10|5|2|703.4|380|78851.0,Agilent6490|2|False|546.29|17|Positive|b3|10|5|2|226.1186|380|68812.0,Agilent6490|3|False|546.29|17|Positive|y8|10|5|2|866.46|380|65056.0,Agilent6490|4|False|546.29|17|Positive|y9|10|5|2|937.5|380|43579.0,Agilent6490|5|False|546.29|17|Positive|y5|10|5|2|533.29|380|43313.0 NA NA NA NA NA NA 8.000000 96.20
P31346 Angiogenin ANG Sus scrofa ATAGFR NA NA PEP20121015013

R+10

R+10 631.32 0.13

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|316.6737|9|Positive|y4|10|5|2|460.2542|380|11135.0,Agilent6490|2|True|316.6737|9|Positive|y3|10|5|2|389.2171|380|4554.0,Agilent6490|3|True|316.6737|9|Positive|y5|10|5|2|561.3019|380|1493.0,Agilent6490|4|True|316.6737|5|Positive|y5++|10|5|2|281.1546|380|1031.0,Agilent6490|5|True|316.6737|9|Positive|y2|10|5|2|332.1956|380|784.0,Agilent6490|1|False|311.669|9|Positive|y4|10|5|2|450.25|380|11135.0,Agilent6490|2|False|311.669|9|Positive|y3|10|5|2|379.21|380|4554.0,Agilent6490|3|False|311.669|9|Positive|y5|10|5|2|551.29|380|1493.0,Agilent6490|4|False|311.669|5|Positive|y5++|10|5|2|276.15|380|1031.0,Agilent6490|5|False|311.669|9|Positive|y2|10|5|2|322.19|380|784.0 3.59(min) 3.59(min);3.600(min);3.35(min) 7.34 7.34;7.34;8.02 View Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4 17.784000 77.80
Q29406 Leptin LEP Sus scrofa NVIQISNDLENLR NA NA PEP20130217017

R+10

R+10 1536.81 -0.42

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|769.4146|17|Positive|b3|10|5|2|327.2032|380|104.0,Agilent6490|4|True|769.4146|25|Positive|b4|10|5|2|455.2618|380|88.0,Agilent6490|5|True|769.4146|41|Positive|y5|10|5|2|654.3814|380|78.0,Agilent6490|2|True|769.4146|21|Positive|y11++|10|5|2|662.8589|380|100.0,Agilent6490|3|True|769.4146|25|Positive|y8|10|5|2|970.4833|380|90.0,Agilent6490|1|False|764.4146|17|Positive|b3|10|5|2|327.2032|380|104.0,Agilent6490|4|False|764.4146|25|Positive|b4|10|5|2|455.2618|380|88.0,Agilent6490|5|False|764.4146|41|Positive|y5|10|5|2|644.3814|380|78.0,Agilent6490|2|False|764.4146|21|Positive|y11++|10|5|2|657.8589|380|100.0,Agilent6490|3|False|764.4146|25|Positive|y8|10|5|2|960.4833|380|90.0 29.890(min) 29.890(min) 20.69 20.69 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 8.450000 96.80
P12682 High mobility group protein B1 HMGB1 Sus scrofa GKPDAAK NA NA PEP2013040904

K+8

K+8 693.38 -1.39

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|347.7029|5|Positive|y5|10|5|2|509.2809|380|3243.0,Agilent6490|2|True|347.7029|13|Positive|y3|10|5|2|297.2012|380|1052.0,Agilent6490|3|True|347.7029|9|Positive|y2|10|5|2|226.1641|380|662.0,Agilent6490|4|True|347.7029|9|Positive|y5++|10|5|2|255.1441|380|537.0,Agilent6490|5|True|347.7029|17|Positive|y4|10|5|2|412.2282|380|476.0,Agilent6490|1|False|343.695|5|Positive|y5|10|5|2|501.27|380|3243.0,Agilent6490|2|False|343.695|13|Positive|y3|10|5|2|289.19|380|1052.0,Agilent6490|3|False|343.695|9|Positive|y2|10|5|2|218.15|380|662.0,Agilent6490|4|False|343.695|9|Positive|y5++|10|5|2|251.14|380|537.0,Agilent6490|5|False|343.695|17|Positive|y4|10|5|2|404.21|380|476.0 NA NA NA NA NA NA 7.000000 59.20
P08059 Glucose-6-phosphate isomerase GPI Sus scrofa VFEGNRPTNSIVFTK NA NA PEP20121015032

K+8

K+8 1715.89 -0.31

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|572.9774|13|Positive|y13++|10|5|3|735.8932|380|109137.0,Agilent6490|2|True|572.9774|13|Positive|y12++|10|5|3|671.3719|380|28304.0,Agilent6490|3|True|572.9774|13|Positive|y14++|10|5|3|809.4274|380|23114.0,Agilent6490|4|True|572.9774|9|Positive|b2|10|5|3|247.1441|380|18015.0,Agilent6490|5|True|572.9774|25|Positive|y2|10|5|3|256.1747|380|12434.0,Agilent6490|1|False|570.3054|13|Positive|y13++|10|5|3|731.886|380|109137.0,Agilent6490|2|False|570.3054|13|Positive|y12++|10|5|3|667.365|380|28304.0,Agilent6490|3|False|570.3054|13|Positive|y14++|10|5|3|805.42|380|23114.0,Agilent6490|4|False|570.3054|9|Positive|b2|10|5|3|247.145|380|18015.0,Agilent6490|5|False|570.3054|25|Positive|y2|10|5|3|248.16|380|12434.0 19.02(min) 19.02(min) 15.96 15.96 View Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4 11.006000 69.83
Q29116 Tenascin TNC Sus scrofa FTTDLDSPR NA NA PEP20120911049

R+10

R+10 1060.5 -0.97

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|531.2611|5|Positive|b2|10|5|2|249.1239|380|2517.0,Agilent6490|2|True|531.2611|25|Positive|y3|10|5|2|369.2126|380|639.0,Agilent6490|4|True|531.2611|17|Positive|y4|10|5|2|484.2395|380|347.0,Agilent6490|5|True|531.2611|29|Positive|y5|10|5|2|597.3236|380|285.0,Agilent6490|3|True|531.2611|17|Positive|y7|10|5|2|813.3982|380|408.0,Agilent6490|1|False|526.2611|5|Positive|b2|10|5|2|249.1239|380|2517.0,Agilent6490|2|False|526.2611|25|Positive|y3|10|5|2|359.2126|380|639.0,Agilent6490|4|False|526.2611|17|Positive|y4|10|5|2|474.2395|380|347.0,Agilent6490|5|False|526.2611|29|Positive|y5|10|5|2|587.3236|380|285.0,Agilent6490|3|False|526.2611|17|Positive|y7|10|5|2|803.3982|380|408.0 13.253(min) 13.253(min);13.0(min);12.49(min);12.65(min);13.253(min);12.18(min);12.180(min) 12.14 12.14;12.01;11.76;13.15;12.14;11.6;11.6 View Gradient 7,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 10,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|1.5|0.4, 1.50|6.3|0.4, 16.00|13.5|0.4, 18.00|13.77|0.4, 33.00|22.5|0.4, 38.00|40.5|0.4, 39.00|81|0.4, 42.90|81|0.4, 43.00|1.5|0.4;Gradient 13,column: Agilent AdvanceBio Peptide Map 2.1 x 150mm 2.7um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 6,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 8.206000 91.70
P62802 Histone H4 NA Sus scrofa DAVTYTEHAK NA NA PEP2013041713

K+8

K+8 1141.54 -0.90

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|381.5243|5|Positive|y6++|10|5|3|378.692|380|141941.0,Agilent6490|2|True|381.5243|5|Positive|y8++|10|5|3|478.75|380|11836.0,Agilent6490|3|True|381.5243|5|Positive|y7++|10|5|3|429.2158|380|10670.0,Agilent6490|4|True|381.5243|13|Positive|y3|10|5|3|363.223|380|3439.0,Agilent6490|5|True|381.5243|9|Positive|y5|10|5|3|593.3133|380|3130.0,Agilent6490|1|False|378.85|5|Positive|y6++|10|5|3|374.68|380|141941.0,Agilent6490|2|False|378.85|5|Positive|y8++|10|5|3|474.74|380|11836.0,Agilent6490|3|False|378.85|5|Positive|y7++|10|5|3|425.21|380|10670.0,Agilent6490|4|False|378.85|13|Positive|y3|10|5|3|355.21|380|3439.0,Agilent6490|5|False|378.85|9|Positive|y5|10|5|3|585.3|380|3130.0 4.270(min) 4.270(min);4.21(min) 7.68 7.68;7.65 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 6.000000 62.10
O19062 C-reactive protein CRP Sus scrofa AFVFPK NA NA PEP999903080004

K+8

K+8 715.4 1.02

Qtrap4000

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|358.7152|5|Positive|y4|10|5|2|498.3171|380|4360.0,Agilent6490|2|True|358.7152|9|Positive|y3|10|5|2|399.2487|380|1234.0,Agilent6490|3|True|358.7152|9|Positive|y5|10|5|2|645.3856|380|593.0,Agilent6490|4|True|358.7152|5|Positive|b3|10|5|2|318.1818|380|271.0,Agilent6490|5|True|358.7152|9|Positive|b5|10|5|2|562.3029|380|62.0,Agilent6490|1|False|354.7152|5|Positive|y4|10|5|2|490.3171|380|4360.0,Agilent6490|2|False|354.7152|9|Positive|y3|10|5|2|391.2487|380|1234.0,Agilent6490|3|False|354.7152|9|Positive|y5|10|5|2|637.3856|380|593.0,Agilent6490|4|False|354.7152|5|Positive|b3|10|5|2|318.1818|380|271.0,Agilent6490|5|False|354.7152|9|Positive|b5|10|5|2|562.3029|380|62.0;Instrument|Rank|Heavy|Q1|Q3|CollisionEnergy|DeclusteringPotential|FragmentIon|ParentChargeState|Time|CellExitPotential|Abundance,Qtrap4000|1|True|358.7|252.2|25|50|y2|2|20|21|0.0,Qtrap4000|2|True|358.7|191.1|21|50|a2|2|20|21|0.0,Qtrap4000|3|True|358.7|498.3|16|50|y4|2|20|21|0.0,Qtrap4000|4|True|358.7|219.1|16|50|b2|2|20|21|0.0,Qtrap4000|5|True|358.7|399.2|21|50|y3|2|20|21|0.0,Qtrap4000|1|False|354.7|244.2|25|50|y2|2|20|21|0.0,Qtrap4000|2|False|354.7|191.1|21|50|a2|2|20|21|0.0,Qtrap4000|3|False|354.7|490.3|16|50|y4|2|20|21|0.0,Qtrap4000|4|False|354.7|219.1|16|50|b2|2|20|21|0.0,Qtrap4000|5|False|354.7|391.2|21|50|y3|2|20|21|0.0 20.9(min) 20.9(min);19.95(min);19.12(min);21.152(min);19.111(min);19.140(min);21.152(min) 15.46 15.46;16.56;14.45;15.6;14.44;14.43;15.6 View Gradient 10,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|1.5|0.4, 1.50|6.3|0.4, 16.00|13.5|0.4, 18.00|13.77|0.4, 33.00|22.5|0.4, 38.00|40.5|0.4, 39.00|81|0.4, 42.90|81|0.4, 43.00|1.5|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 6,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 9,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|2.7|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.8|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 7,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4 8.580000 98.70
Q8SPS7 Haptoglobin HP Sus scrofa VGYVSGWGR NA NA PEP9999040956

R+6

R+6 985.49 -0.03

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|493.751|13|Positive|y5|10|5|2|568.282|380|778.0,Agilent6490|2|True|493.751|13|Positive|b3|10|5|2|320.161|380|511.0,Agilent6490|3|True|493.751|17|Positive|y6|10|5|2|667.3505|380|286.0,Agilent6490|4|True|493.751|9|Positive|y4|10|5|2|481.25|380|274.0,Agilent6490|5|True|493.751|9|Positive|y7++|10|5|2|415.7108|380|256.0,Agilent6490|1|False|490.7557|13|Positive|y5|10|5|2|562.282|380|778.0,Agilent6490|2|False|490.7557|13|Positive|b3|10|5|2|320.161|380|511.0,Agilent6490|3|False|490.7557|17|Positive|y6|10|5|2|661.3505|380|286.0,Agilent6490|4|False|490.7557|9|Positive|y4|10|5|2|475.25|380|274.0,Agilent6490|5|False|490.7557|9|Positive|y7++|10|5|2|412.7108|380|256.0 NA NA NA NA NA NA 8.600000 85.10
P16611 Insulin-like growth factor-binding protein 3 IGFBP3 Sus scrofa PLQALLDGR NA NA PEP9999032509

R+10

R+10 991.56 -0.03

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|496.7923|13|Positive|b3|10|5|2|339.2032|380|584.0,Agilent6490|2|True|496.7923|17|Positive|y7|10|5|2|782.44|380|483.0,Agilent6490|3|True|496.7923|13|Positive|y6|10|5|2|654.3814|380|482.0,Agilent6490|4|True|496.7923|17|Positive|y4|10|5|2|470.2602|380|407.0,Agilent6490|5|True|496.7923|17|Positive|y5|10|5|2|583.3443|380|290.0,Agilent6490|1|False|491.7923|13|Positive|b3|10|5|2|339.2032|380|584.0,Agilent6490|2|False|491.7923|17|Positive|y7|10|5|2|772.44|380|483.0,Agilent6490|3|False|491.7923|13|Positive|y6|10|5|2|644.3814|380|482.0,Agilent6490|4|False|491.7923|17|Positive|y4|10|5|2|460.2602|380|407.0,Agilent6490|5|False|491.7923|17|Positive|y5|10|5|2|573.3443|380|290.0 21.98(min) 21.98(min);21.21(min);21.290(min) 17.87 17.87;15.66;15.68 View Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 6.990000 100.00
Q5S1U1 Heat shock protein beta-1 HSPB1 Sus scrofa LFDQAFGLPR NA NA PEP2013092504

R+10

R+10 1172.61 0.15

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|587.3187|21|Positive|y8|10|5|2|913.4766|380|208719.0,Agilent6490|2|True|587.3187|25|Positive|y5|10|5|2|599.3539|380|121825.0,Agilent6490|3|True|587.3187|17|Positive|y2|10|5|2|282.18|380|93099.0,Agilent6490|4|True|587.3187|13|Positive|y4|10|5|2|452.2855|380|91679.0,Agilent6490|5|True|587.3187|17|Positive|y6|10|5|2|670.391|380|87185.0,Agilent6490|1|False|582.31|21|Positive|y8|10|5|2|903.47|380|208719.0,Agilent6490|2|False|582.31|25|Positive|y5|10|5|2|589.35|380|121825.0,Agilent6490|3|False|582.31|17|Positive|y2|10|5|2|272.17|380|93099.0,Agilent6490|4|False|582.31|13|Positive|y4|10|5|2|442.28|380|91679.0,Agilent6490|5|False|582.31|17|Positive|y6|10|5|2|660.38|380|87185.0 NA NA NA NA NA NA 8.000000 98.40
P53027 60S ribosomal protein L10a RPL10A Sus scrofa ILGPGLNK NA NA PEP2016062214

K+8

K+8 818.5 0.29

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|410.2631|5|Positive|b2|10|5|2|227.1754|380|593920.0,Agilent6490|2|True|410.2631|9|Positive|y6|10|5|2|593.3497|380|281489.0,Agilent6490|3|True|410.2631|9|Positive|y7|10|5|2|706.4338|380|59346.0,Agilent6490|4|True|410.2631|13|Positive|y5|10|5|2|536.3282|380|27607.0,Agilent6490|5|True|410.2631|17|Positive|y6++|10|5|2|297.1785|380|24835.0,Agilent6490|1|False|406.26|5|Positive|b2|10|5|2|227.1754|380|593920.0,Agilent6490|2|False|406.26|9|Positive|y6|10|5|2|585.33|380|281489.0,Agilent6490|3|False|406.26|9|Positive|y7|10|5|2|698.42|380|59346.0,Agilent6490|4|False|406.26|13|Positive|y5|10|5|2|528.31|380|27607.0,Agilent6490|5|False|406.26|17|Positive|y6++|10|5|2|293.17|380|24835.0 NA NA NA NA NA NA 7.900000 96.33
P24853 Insulin-like growth factor-binding protein 2 IGFBP2 Sus scrofa LIQGAPTIR NA NA PEP2013040220

R+10

R+10 977.58 0.43

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|489.8027|9|Positive|y7++|10|5|2|376.7181|380|159178.0,Agilent6490|2|True|489.8027|13|Positive|y6|10|5|2|624.3703|380|130564.0,Agilent6490|3|True|489.8027|13|Positive|y7|10|5|2|752.4289|380|128208.0,Agilent6490|4|True|489.8027|9|Positive|b2|10|5|2|227.1754|380|72690.0,Agilent6490|5|True|489.8027|9|Positive|y4|10|5|2|496.3117|380|57554.0,Agilent6490|1|False|484.797|9|Positive|y7++|10|5|2|371.71|380|159178.0,Agilent6490|2|False|484.797|13|Positive|y6|10|5|2|614.36|380|130564.0,Agilent6490|3|False|484.797|13|Positive|y7|10|5|2|742.42|380|128208.0,Agilent6490|4|False|484.797|9|Positive|b2|10|5|2|227.1754|380|72690.0,Agilent6490|5|False|484.797|9|Positive|y4|10|5|2|486.3|380|57554.0 15.0(min) 15.0(min);14.53(min);15.203(min);13.94(min);15.203(min);13.900(min) 13 13;12.77;13.1;12.48;13.1;12.46 View Gradient 10,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|1.5|0.4, 1.50|6.3|0.4, 16.00|13.5|0.4, 18.00|13.77|0.4, 33.00|22.5|0.4, 38.00|40.5|0.4, 39.00|81|0.4, 42.90|81|0.4, 43.00|1.5|0.4;Gradient 13,column: Agilent AdvanceBio Peptide Map 2.1 x 150mm 2.7um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 6,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 7,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 13.000000 100.00
P12068 Lysozyme C-2 NA Sus scrofa AWVAWR NA NA PEP20121119013

R+10

R+10 797.41 0.25

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|2|True|399.7184|9|Positive|b2|10|5|2|258.1237|380|26838.0,Agilent6490|2|False|394.7137|9|Positive|b2|10|5|2|258.124|380|26838.0,Agilent6490|5|True|399.7184|9|Positive|b3|10|5|2|357.1921|380|3732.0,Agilent6490|5|False|394.7137|9|Positive|b3|10|5|2|357.193|380|3732.0,Agilent6490|3|True|399.7184|13|Positive|y3|10|5|2|442.2436|380|16662.0,Agilent6490|3|False|394.7137|13|Positive|y3|10|5|2|432.235|380|16662.0,Agilent6490|1|True|399.7184|9|Positive|y4|10|5|2|541.3121|380|67309.0,Agilent6490|1|False|394.7137|9|Positive|y4|10|5|2|531.303|380|67309.0,Agilent6490|4|True|399.7184|9|Positive|y5|10|5|2|364.1993|380|3922.0,Agilent6490|4|False|394.7137|9|Positive|y5|10|5|2|359.195|380|3922.0 20.9(min) 20.9(min);21.77(min);21.77(min);22.476(min);22.476(min);20.930(min) 15.46 15.46;17.74;17.74;16.38;16.38;15.48 View Gradient 10,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|1.5|0.4, 1.50|6.3|0.4, 16.00|13.5|0.4, 18.00|13.77|0.4, 33.00|22.5|0.4, 38.00|40.5|0.4, 39.00|81|0.4, 42.90|81|0.4, 43.00|1.5|0.4;Gradient 11,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 7,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 6,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 8.968000 93.50
P67937 Tropomyosin alpha-4 chain TPM4 Sus scrofa TIDDLEEK NA NA PEP20171206025

K+8

K+8 969.46 -1.29

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|485.7451|9|Positive|y6|10|5|2|756.3501|380|100641.0,Agilent6490|2|True|485.7451|9|Positive|b2|10|5|2|215.139|380|61060.0,Agilent6490|3|True|485.7451|21|Positive|y2|10|5|2|284.1696|380|13446.0,Agilent6490|4|True|485.7451|21|Positive|b6++|10|5|2|344.1634|380|12414.0,Agilent6490|5|True|485.7451|17|Positive|y4|10|5|2|526.2963|380|9885.0,Agilent6490|1|False|481.7375|9|Positive|y6|10|5|2|748.335|380|100641.0,Agilent6490|2|False|481.7375|9|Positive|b2|10|5|2|215.14|380|61060.0,Agilent6490|3|False|481.7375|21|Positive|y2|10|5|2|276.155|380|13446.0,Agilent6490|4|False|481.7375|21|Positive|b6++|10|5|2|344.16|380|12414.0,Agilent6490|5|False|481.7375|17|Positive|y4|10|5|2|518.281|380|9885.0 NA NA NA NA NA NA 9.330000 77.15
Q5XLD3 Creatine kinase M-type CKM Sus scrofa FEEILTR NA NA PEP999903080009

R+10

R+10 916.48 -0.16

Qtrap4000

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|2|True|459.2525|13|Positive|y3|10|5|2|399.2595|380|536.0,Agilent6490|4|True|459.2525|13|Positive|b3|10|5|2|406.1614|380|384.0,Agilent6490|3|True|459.2525|13|Positive|y4|10|5|2|512.3436|380|521.0,Agilent6490|1|True|459.2525|13|Positive|y5|10|5|2|641.3862|380|1173.0,Agilent6490|5|True|459.2525|17|Positive|y6|10|5|2|770.4288|380|302.0,Agilent6490|2|False|454.2525|13|Positive|y3|10|5|2|389.2595|380|536.0,Agilent6490|4|False|454.2525|13|Positive|b3|10|5|2|406.1614|380|384.0,Agilent6490|3|False|454.2525|13|Positive|y4|10|5|2|502.3436|380|521.0,Agilent6490|1|False|454.2525|13|Positive|y5|10|5|2|631.3862|380|1173.0,Agilent6490|5|False|454.2525|17|Positive|y6|10|5|2|760.4288|380|302.0;Instrument|Rank|Heavy|Q1|Q3|CollisionEnergy|DeclusteringPotential|FragmentIon|ParentChargeState|Time|CellExitPotential|Abundance,Qtrap4000|1|True|459.2|641.4|23|65|y5|2|20|21|0.0,Qtrap4000|2|True|459.2|277.1|23|65|b2|2|20|21|0.0,Qtrap4000|3|True|459.2|249.1|27|65|a2|2|20|21|0.0,Qtrap4000|4|True|459.2|512.3|26|65|y4|2|20|21|0.0,Qtrap4000|5|True|459.2|770.4|26|65|y6|2|20|21|0.0,Qtrap4000|1|False|454.2|631.4|23|65|y5|2|20|21|0.0,Qtrap4000|2|False|454.2|277.1|23|65|b2|2|20|21|0.0,Qtrap4000|3|False|454.2|249.1|27|65|a2|2|20|21|0.0,Qtrap4000|4|False|454.2|502.3|26|65|y4|2|20|21|0.0,Qtrap4000|5|False|454.2|760.4|26|65|y6|2|20|21|0.0 15.900(min) 15.900(min);17.249(min);16.46(min);15.88(min);17.249(min);17.0(min) 13.45 13.45;13.67;15.07;13.44;13.67;13.63 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 7,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 6,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 10,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|1.5|0.4, 1.50|6.3|0.4, 16.00|13.5|0.4, 18.00|13.77|0.4, 33.00|22.5|0.4, 38.00|40.5|0.4, 39.00|81|0.4, 42.90|81|0.4, 43.00|1.5|0.4 6.160000 94.10
P62802 Histone H4 NA Sus scrofa VFLENVIR NA NA PEP2013040914

R+10

R+10 998.57 1.00

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|5|True|500.2972|13|Positive|y7|10|5|2|900.5177|380|5368.0,Agilent6490|4|True|500.2972|13|Positive|y4|10|5|2|511.3226|380|5863.0,Agilent6490|3|True|500.2972|9|Positive|y5|10|5|2|640.3652|380|15110.0,Agilent6490|2|True|500.2972|9|Positive|b2|10|5|2|247.1441|380|25536.0,Agilent6490|1|True|500.2972|9|Positive|y6|10|5|2|753.4493|380|33126.0,Agilent6490|5|False|495.2925|13|Positive|y7|10|5|2|890.51|380|5368.0,Agilent6490|4|False|495.2925|13|Positive|y4|10|5|2|501.31|380|5863.0,Agilent6490|3|False|495.2925|9|Positive|y5|10|5|2|630.36|380|15110.0,Agilent6490|2|False|495.2925|9|Positive|b2|10|5|2|247.1441|380|25536.0,Agilent6490|1|False|495.2925|9|Positive|y6|10|5|2|743.44|380|33126.0 26.810(min) 26.810(min) 18.9 18.9 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 11.000000 100.00
A5D9M6 Large proline-rich protein BAG6 BAG6 Sus scrofa LLGNTFVALSDLR NA NA PEP2013101029

R+10

R+10 1427.79 0.82

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|714.9084|37|Positive|y2|10|5|2|298.2113|380|179493.0,Agilent6490|2|True|714.9084|25|Positive|y7|10|5|2|783.4599|380|112731.0,Agilent6490|3|True|714.9084|21|Positive|y6|10|5|2|684.3914|380|94654.0,Agilent6490|4|True|714.9084|21|Positive|b2|10|5|2|227.1754|380|90303.0,Agilent6490|5|True|714.9084|21|Positive|y8|10|5|2|930.5283|380|69880.0,Agilent6490|1|False|709.9|37|Positive|y2|10|5|2|288.2|380|179493.0,Agilent6490|2|False|709.9|25|Positive|y7|10|5|2|773.45|380|112731.0,Agilent6490|3|False|709.9|21|Positive|y6|10|5|2|674.38|380|94654.0,Agilent6490|4|False|709.9|21|Positive|b2|10|5|2|227.1754|380|90303.0,Agilent6490|5|False|709.9|21|Positive|y8|10|5|2|920.52|380|69880.0 35.420(min) 35.420(min) 31.21 31.21 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 NA 95.17
P23563 Tumor necrosis factor TNF Sus scrofa IAVSYQTK NA NA PEP20120911083

K+8

K+8 916.5 0.04

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|459.26|5|Positive|b3|10|5|2|284.2|380|2774.0,Agilent6490|2|True|459.26|13|Positive|y6|10|5|2|733.4|380|1205.0,Agilent6490|3|True|459.26|9|Positive|y5|10|5|2|634.33|380|1138.0,Agilent6490|4|True|459.26|21|Positive|y2|10|5|2|256.18|380|543.0,Agilent6490|5|True|459.26|13|Positive|y7|10|5|2|804.43|380|501.0,Agilent6490|1|False|455.26|5|Positive|b3|10|5|2|284.2|380|2774.0,Agilent6490|2|False|455.26|13|Positive|y6|10|5|2|725.4|380|1205.0,Agilent6490|3|False|455.26|9|Positive|y5|10|5|2|626.33|380|1138.0,Agilent6490|4|False|455.26|21|Positive|y2|10|5|2|248.18|380|543.0,Agilent6490|5|False|455.26|13|Positive|y7|10|5|2|796.43|380|501.0 7.790(min) 7.790(min);7.79(min);7.75(min);8.00(min) 9.42 9.42;9.42;9.4;10.59 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 3,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|1.5|0.4, 1.50|6.3|0.4, 16.00|13.5|0.4, 18.00|13.77|0.4, 33.00|22.5|0.4, 38.00|40.5|0.4, 39.00|81|0.4, 42.90|81|0.4, 43.00|1.5|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4 7.522000 90.40
P28491 Calreticulin CALR Sus scrofa GQTLVVQFTVK NA NA PEP20130206010

K+8

K+8 1226.7 0.59

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|614.3635|21|Positive|y7|10|5|2|828.5069|380|6883.0,Agilent6490|2|True|614.3635|13|Positive|y6|10|5|2|729.4385|380|6814.0,Agilent6490|3|True|614.3635|17|Positive|y9|10|5|2|1042.6387|380|4717.0,Agilent6490|4|True|614.3635|13|Positive|y8|10|5|2|941.591|380|4048.0,Agilent6490|5|True|614.3635|13|Positive|b3|10|5|2|287.135|380|3341.0,Agilent6490|1|False|610.36|21|Positive|y7|10|5|2|820.49|380|6883.0,Agilent6490|2|False|610.36|13|Positive|y6|10|5|2|721.42|380|6814.0,Agilent6490|3|False|610.36|17|Positive|y9|10|5|2|1034.62|380|4717.0,Agilent6490|4|False|610.36|13|Positive|y8|10|5|2|933.58|380|4048.0,Agilent6490|5|False|610.36|13|Positive|b3|10|5|2|287.135|380|3341.0 26.650(min) 26.650(min) 18.8 18.8 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 12.000000 92.20
P04404 Chromogranin-A CHGA Sus scrofa RPEDQELESLSAIEAELEK NA NA PEP2013021909

K+8

K+8 2193.08 -1.06

Qtrap4000

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|732.0376|17|Positive|y9++|10|5|3|499.2688|380|358.0,Agilent6490|2|True|732.0376|13|Positive|b13++|10|5|3|734.8681|380|290.0,Agilent6490|3|True|732.0376|13|Positive|y11++|10|5|3|599.3268|380|289.0,Agilent6490|4|True|732.0376|17|Positive|b9++|10|5|3|542.7495|380|274.0,Agilent6490|5|True|732.0376|13|Positive|b11++|10|5|3|642.8075|380|246.0,Agilent6490|1|False|729.370933333|17|Positive|y9++|10|5|3|495.2688|380|358.0,Agilent6490|2|False|729.370933333|13|Positive|b13++|10|5|3|734.8681|380|290.0,Agilent6490|3|False|729.370933333|13|Positive|y11++|10|5|3|595.3268|380|289.0,Agilent6490|4|False|729.370933333|17|Positive|b9++|10|5|3|542.7495|380|274.0,Agilent6490|5|False|729.370933333|13|Positive|b11++|10|5|3|642.8075|380|246.0;Instrument|Rank|Heavy|Q1|Q3|CollisionEnergy|DeclusteringPotential|FragmentIon|ParentChargeState|Time|CellExitPotential|Abundance,Qtrap4000|1|True|732.0|726.4|27|65|y6|3|20|21|0.0,Qtrap4000|2|True|732.0|839.5|27|65|y7|3|20|21|0.0,Qtrap4000|3|True|732.0|755.3|45|65|b6|3|20|21|0.0,Qtrap4000|4|True|732.0|599.3|25|65|y++11|3|20|21|0.0,Qtrap4000|5|True|732.0|597.3|26|65|y5|3|20|21|0.0,Qtrap4000|1|False|729.4|718.4|27|65|y6|3|20|21|0.0,Qtrap4000|2|False|729.4|831.5|27|65|y7|3|20|21|0.0,Qtrap4000|3|False|729.4|755.3|45|65|b6|3|20|21|0.0,Qtrap4000|4|False|729.4|595.3|25|65|y++11|3|20|21|0.0,Qtrap4000|5|False|729.4|589.3|26|65|y5|3|20|21|0.0 35.21(min) 35.21(min) 33.84 33.84 View Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4 6.520000 98.70
P23687 Prolyl endopeptidase PREP Sus scrofa VLYVQDSLEGEAR NA NA PEP2013101019

R+10

R+10 1487.74 -0.25

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|744.8826|29|Positive|b2|10|5|2|213.1598|380|54975.0,Agilent6490|2|True|744.8826|21|Positive|b3|10|5|2|376.2231|380|44146.0,Agilent6490|3|True|744.8826|25|Positive|y9|10|5|2|1014.4726|380|42309.0,Agilent6490|4|True|744.8826|21|Positive|y11++|10|5|2|638.8058|380|31159.0,Agilent6490|5|True|744.8826|37|Positive|y2|10|5|2|256.1643|380|30241.0,Agilent6490|1|False|739.8826|29|Positive|b2|10|5|2|213.1598|380|54975.0,Agilent6490|2|False|739.8826|21|Positive|b3|10|5|2|376.2231|380|44146.0,Agilent6490|3|False|739.8826|25|Positive|y9|10|5|2|1004.4726|380|42309.0,Agilent6490|4|False|739.8826|21|Positive|y11++|10|5|2|633.8058|380|31159.0,Agilent6490|5|False|739.8826|37|Positive|y2|10|5|2|246.1643|380|30241.0 21.130(min) 21.130(min);21.08(min) 15.59 15.59;15.59 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4 2.600000 79.21
P20305-2 Gelsolin GSN Sus scrofa GGVASGFK NA NA PEP2013091939

K+8

K+8 729.38 0.36

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|365.7029|9|Positive|y5|10|5|2|517.286|380|506949.0,Agilent6490|2|True|365.7029|9|Positive|y4|10|5|2|446.2489|380|165343.0,Agilent6490|3|True|365.7029|5|Positive|y6++|10|5|2|308.6809|380|71020.0,Agilent6490|4|True|365.7029|9|Positive|y6|10|5|2|616.3544|380|43362.0,Agilent6490|5|True|365.7029|13|Positive|y2|10|5|2|302.1954|380|37892.0,Agilent6490|1|False|361.7|9|Positive|y5|10|5|2|509.27|380|506949.0,Agilent6490|2|False|361.7|9|Positive|y4|10|5|2|438.23|380|165343.0,Agilent6490|3|False|361.7|5|Positive|y6++|10|5|2|304.67|380|71020.0,Agilent6490|4|False|361.7|9|Positive|y6|10|5|2|608.34|380|43362.0,Agilent6490|5|False|361.7|13|Positive|y2|10|5|2|294.18|380|37892.0 6.11(min) 6.11(min);5.64(min);5.650(min) 8.59 8.59;8.36;8.36 View Gradient 13,column: Agilent AdvanceBio Peptide Map 2.1 x 150mm 2.7um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 10.000000 84.29
Q29406 Leptin LEP Sus scrofa NVIQISNDLENLR NA NA PEP9999042016

R+10

R+10 1536.81 -0.42

Qtrap4000

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|769.4146|17|Positive|b3|10|5|2|327.2032|380|104.0,Agilent6490|4|True|769.4146|25|Positive|b4|10|5|2|455.2618|380|88.0,Agilent6490|5|True|769.4146|41|Positive|y5|10|5|2|654.3814|380|78.0,Agilent6490|2|True|769.4146|21|Positive|y11++|10|5|2|662.8589|380|100.0,Agilent6490|3|True|769.4146|25|Positive|y8|10|5|2|970.4833|380|90.0,Agilent6490|1|False|764.4146|17|Positive|b3|10|5|2|327.2032|380|104.0,Agilent6490|4|False|764.4146|25|Positive|b4|10|5|2|455.2618|380|88.0,Agilent6490|5|False|764.4146|41|Positive|y5|10|5|2|644.3814|380|78.0,Agilent6490|2|False|764.4146|21|Positive|y11++|10|5|2|657.8589|380|100.0,Agilent6490|3|False|764.4146|25|Positive|y8|10|5|2|960.4833|380|90.0;Instrument|Rank|Heavy|Q1|Q3|CollisionEnergy|DeclusteringPotential|FragmentIon|ParentChargeState|Time|CellExitPotential|Abundance,Qtrap4000|1|True|513.3|541.3|23|57|y4|3|20|21|0.0,Qtrap4000|2|True|513.3|662.9|17|57|y++11|3|20|21|0.0,Qtrap4000|3|True|513.3|884.5|20|57|b8|3|20|21|0.0,Qtrap4000|4|True|513.3|654.4|23|57|y5|3|20|21|0.0,Qtrap4000|5|True|513.3|412.3|25|57|y3|3|20|21|0.0,Qtrap4000|1|False|509.9|531.3|23|57|y4|3|20|21|0.0,Qtrap4000|2|False|509.9|657.9|17|57|y++11|3|20|21|0.0,Qtrap4000|3|False|509.9|884.5|20|57|b8|3|20|21|0.0,Qtrap4000|4|False|509.9|644.4|23|57|y5|3|20|21|0.0,Qtrap4000|5|False|509.9|402.3|25|57|y3|3|20|21|0.0 NA NA NA NA NA NA 6.310000 92.50
P12067 Lysozyme C-1 NA Sus scrofa AWVAWR NA NA PEP20121119013

R+10

R+10 797.41 0.25

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|2|True|399.7184|9|Positive|b2|10|5|2|258.1237|380|26838.0,Agilent6490|2|False|394.7137|9|Positive|b2|10|5|2|258.124|380|26838.0,Agilent6490|5|True|399.7184|9|Positive|b3|10|5|2|357.1921|380|3732.0,Agilent6490|5|False|394.7137|9|Positive|b3|10|5|2|357.193|380|3732.0,Agilent6490|3|True|399.7184|13|Positive|y3|10|5|2|442.2436|380|16662.0,Agilent6490|3|False|394.7137|13|Positive|y3|10|5|2|432.235|380|16662.0,Agilent6490|1|True|399.7184|9|Positive|y4|10|5|2|541.3121|380|67309.0,Agilent6490|1|False|394.7137|9|Positive|y4|10|5|2|531.303|380|67309.0,Agilent6490|4|True|399.7184|9|Positive|y5|10|5|2|364.1993|380|3922.0,Agilent6490|4|False|394.7137|9|Positive|y5|10|5|2|359.195|380|3922.0 20.9(min) 20.9(min);21.77(min);21.77(min);22.476(min);22.476(min);20.930(min) 15.46 15.46;17.74;17.74;16.38;16.38;15.48 View Gradient 10,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|1.5|0.4, 1.50|6.3|0.4, 16.00|13.5|0.4, 18.00|13.77|0.4, 33.00|22.5|0.4, 38.00|40.5|0.4, 39.00|81|0.4, 42.90|81|0.4, 43.00|1.5|0.4;Gradient 11,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4;Gradient 7,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 6,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|2.7|0.4;Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4 8.968000 93.50
P68137 Actin, alpha skeletal muscle ACTA1 Sus scrofa GYSFVTTAER NA NA PEP20120911016

R+10

R+10 1139.54 -0.31

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|570.7822|5|Positive|b2|10|5|2|221.0926|380|2501.0,Agilent6490|4|True|570.7822|17|Positive|b3|10|5|2|308.1246|380|589.0,Agilent6490|2|True|570.7822|21|Positive|y5|10|5|2|587.3028|380|1117.0,Agilent6490|3|True|570.7822|13|Positive|y6|10|5|2|686.3713|380|797.0,Agilent6490|5|True|570.7822|5|Positive|y8++|10|5|2|460.7398|380|534.0,Agilent6490|1|False|565.7822|5|Positive|b2|10|5|2|221.0926|380|2501.0,Agilent6490|4|False|565.7822|17|Positive|b3|10|5|2|308.1246|380|589.0,Agilent6490|2|False|565.7822|21|Positive|y5|10|5|2|577.3028|380|1117.0,Agilent6490|3|False|565.7822|13|Positive|y6|10|5|2|676.3713|380|797.0,Agilent6490|5|False|565.7822|5|Positive|y8++|10|5|2|455.7398|380|534.0 NA NA NA NA NA NA 10.538000 100.00
P26234 Vinculin VCL Sus scrofa ALASQLQDSLK NA NA PEP2016051322

K+8

K+8 1180.64 -0.09

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|591.335|21|Positive|y9|10|5|2|997.5404|380|527896.0,Agilent6490|2|True|591.335|17|Positive|y8|10|5|2|926.5033|380|416680.0,Agilent6490|3|True|591.335|13|Positive|y9++|10|5|2|499.2738|380|349294.0,Agilent6490|4|True|591.335|17|Positive|b3|10|5|2|256.1656|380|224785.0,Agilent6490|5|True|591.335|17|Positive|y5|10|5|2|598.3286|380|147788.0,Agilent6490|1|False|587.33|21|Positive|y9|10|5|2|989.53|380|527896.0,Agilent6490|2|False|587.33|17|Positive|y8|10|5|2|918.49|380|416680.0,Agilent6490|3|False|587.33|13|Positive|y9++|10|5|2|495.27|380|349294.0,Agilent6490|4|False|587.33|17|Positive|b3|10|5|2|256.1656|380|224785.0,Agilent6490|5|False|587.33|17|Positive|y5|10|5|2|590.31|380|147788.0 NA NA NA NA NA NA 8.200000 98.27
Q767M3 Valine--tRNA ligase, mitochondrial VARS2 Sus scrofa LSSLQLELSK NA NA PEP20121207029

K+8

K+8 1124.64 0.19

Agilent6490

Instrument|Rank|Heavy|PrecursorIon|CollisionEnergy|Polarity|FragmentIon|Dwell|CellAcceleratorVoltage|ParentChargeState|ProductIon|Fragmentor|Abundance,Agilent6490|1|True|563.3344|25|Positive|y4|10|5|2|484.2857|380|9292.0,Agilent6490|2|True|563.3344|13|Positive|y6|10|5|2|725.4283|380|5552.0,Agilent6490|3|True|563.3344|21|Positive|y9|10|5|2|1012.5765|380|5464.0,Agilent6490|4|True|563.3344|33|Positive|b4++|10|5|2|201.1234|380|5086.0,Agilent6490|5|True|563.3344|29|Positive|b3|10|5|2|288.1554|380|2871.0,Agilent6490|1|False|559.3268|25|Positive|y4|10|5|2|476.27|380|9292.0,Agilent6490|2|False|559.3268|13|Positive|y6|10|5|2|717.41|380|5552.0,Agilent6490|3|False|559.3268|21|Positive|y9|10|5|2|1004.56|380|5464.0,Agilent6490|4|False|559.3268|33|Positive|b4++|10|5|2|201.12|380|5086.0,Agilent6490|5|False|559.3268|29|Positive|b3|10|5|2|288.16|380|2871.0 20.540(min) 20.540(min);20.55(min);21.30(min) 15.25 15.25;15.28;17.43 View Gradient 2,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0|1.5|0.4, 1.5|6.3|0.4, 16|13.5|0.4, 18|13.77|0.4, 33|22.5|0.4, 38|40.5|0.4, 39|81|0.4, 42.9|81|0.4, 43|1.5|0.4;Gradient 4,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|2.70|0.4, 1.50|6.30|0.4, 16.00|13.50|0.4, 18.00|13.80|0.4, 33.00|22.50|0.4, 38.00|40.50|0.4, 39.00|81.00|0.4, 42.90|81.00|0.4, 43.00|2.70|0.4;Gradient 1,column: Agilent Zorbax Eclipse Plus C18 RRHD 2.1 x 150mm 1.8um, Time [min] | B [%] | Flow [ml/min], 0.00|3.00|0.4, 1.50|7.00|0.4, 16.00|15.00|0.4, 18.00|15.30|0.4, 33.00|25.00|0.4, 38.00|45.00|0.4, 39.00|90.00|0.4, 42.90|90.00|0.4, 43.00|3.00|0.4 10.221000 77.30